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Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (9): 2554-2564.doi: 10.16420/j.issn.0513-353x.2024-0849

• New Technology and New Method • Previous Articles     Next Articles

Optimization of Paraffin Section Preparation Method for Pear Tissues

WU Chunhao, YAN Xingkai, LU Mingyan, ZHAO Ying, ZHANG Maojun, LI Siyu, WANG Qiang*()   

  1. Jilin Academy of Agricultural Sciences(Northeast China Agricultural Science and Technology Innovation Center)Institute of Pomology/Northeast China(Jilin)Fruit ScienceGongzhuling, Jilin 136100, China
  • Received:2025-03-17 Revised:2025-07-07 Online:2025-09-25 Published:2025-09-24
  • Contact: WANG Qiang

Abstract:

Based on the conventional paraffin section method,using pear leaves,shoots,and flower buds as experimental materials,optimized key steps—including dehydration,clearing,pasting and staining. The results demonstrated that employing microwave and water bath processing dramatically reduced the total processing time(encompassing dehydration,clearing and dipping paraffin)from 750-1 320 min to 48-108 min. Screening of three clearing agent revealed that the clearing agent used for dipping paraffin and dewaxing solution demonstrated superior clearing efficacy,while significantly reducing xylene usage. The water bath mounting method combined tissue flattening and adhesion into a single step,simplifying the procedure. Comparative evaluation of four staining methods allowed selection of the most suitable protocol based on specific sectioning requirements. Ultimately,developed an integrated microwave and water bath paraffin sectioning method. This method significantly shortens preparation time and enhances sectioning efficiency.

Key words: pear, paraffin section method, microwave and water bath method