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Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (9): 2532-2544.doi: 10.16420/j.issn.0513-353x.2024-0811

• Plant Protection • Previous Articles     Next Articles

Isolation,Biological Characteristics of the Pathogen Causing Fusarium Wilt on Tomato and Antagonistic Fungus Screening

SU Xiumin1,*(), WANG Jiao1, HAN Wenqing1, LI Peng2, WANG Qiulan1, LI Wanxing1   

  1. 1 Institute of Millet of Shanxi Agricultural UniversityChangzhi, Shanxi 046000, China
    2 Ministry of Agriculture and Rural Affairs of LuzhouChangzhi, Shanxi 046000, China
  • Received:2024-12-02 Revised:2025-06-05 Online:2025-09-25 Published:2025-09-24
  • Contact: SU Xiumin

Abstract:

Tomato Fusarium wilt samples from the stems in Huguan County,Changzhi City,Shanxi Province,were used as experimental materials. The pathogen was isolated and cultured using tissue separation methods,and its pathogenicity was determined by root-dipping inoculation. The pathogen species responsible for the tomato wilt disease was identified using conventional morphological analysis and ITS sequence analysis. Additionally,the biological characteristics of the pathogen were studied. Furthermore,after two rounds of tomato-zucchini crop rotation,Trichoderma harzianum strain M408 was isolated from the soil planted with zucchini. The antagonistic activity of Trichoderma harzianum M408 mycelium and its sterile fermentation liquid against the tomato wilt pathogen was measured using the dual-culture plate method. The results revealed that the pathogen causing tomato wilt disease in Huguan County,Changzhi City,Shanxi Province,was identified as Fusarium proliferatum,named K11. The suitable growth temperature for K11 was 28 ℃,and 6.0 was the most suitable pH value. Under 24-hour full light conditions,the growth rate of K11 was fastest. K11 was able to grow using various carbon sources,with the fastest mycelial growth observed when sucrose was used as the carbon source. Using the mycelial confrontation method on plates,after 7 days,Trichoderma harzianum M408 showed a 68.38% inhibition rate on the K11 mycelium,and its sterile fermentation liquid exhibited 93.17% inhibition rate against K11 mycelium.

Key words: tomato, Fusarium wilt, pathogen identification, Fusarium proliferatum, biological characteristics, antagonistic fungus