Effect of Exogenous Strigolactones on the Secondary Growth of Poncirus trifoliata Seedlings Stem

doi:10.16420/j.issn.0513-353x.2024-0528

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (7): 1843-1854.doi: 10.16420/j.issn.0513-353x.2024-0528

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Effect of Exogenous Strigolactones on the Secondary Growth of Poncirus trifoliata Seedlings Stem

ZHOU Tian, LÜ Shuaili, HAN Han, LI Yujia, LIU Haiqiang, and LIU Yongzhong^*()

National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops，Horticulture & Forestry Sciences of Huazhong Agricultural University，Wuhan 430070，China

Received:2025-02-23 Revised:2025-04-18 Online:2025-07-23 Published:2025-07-23
Contact: and LIU Yongzhong

Abstract

Abstract:

Applying varying concentrations of strigolactones（SLs）analogue GR24（0，10，20，30 μmol · L^-1），growth，anatomical structures，and physiological-biochemical changes were assessed，along with the analysis of gene expression via Quantitative Real-time PCR. The result indicated that stem thickening growth was concentration-dependent，only with the 20 μmol · L^-1 treatment significantly enhanced it. It was characterized by increased xylem width，the thickness of secondary cell wall thickening，the content of lignin，cellulose，and hemicellulos. The level of endogenous hormones，including SLs，auxin and gibberellins，also increased. qRT-PCR revealed significantly upregulated expression of auxin-responsive genes（ARF5），xylem-related genes（KNAT1，STM，CAD），and cambium-specific genes （WOX4），indicating that the 20 μmol · L^-1 GR24 treatment enhanced thickening by promoting secondary xylem cell proliferation and lignification，and inducing hormone increases.

Key words: citrus, Poncirus trifoliata, strigolactones, secondary growth, expression of related genes

ZHOU Tian, LÜ Shuaili, HAN Han, LI Yujia, LIU Haiqiang, and LIU Yongzhong. Effect of Exogenous Strigolactones on the Secondary Growth of Poncirus trifoliata Seedlings Stem[J]. Acta Horticulturae Sinica, 2025, 52(7): 1843-1854.

Figures/Tables 9

Fig. 1 Effect of different concentrations of GR24 on Poncirus trifoliata seedlings ground diameter and plant height Different lowercase letters at the same time indicate significant difference at P ≤ 0.05. The same in the following

Table 1 Hormones-related and secondary growth-related genes in Poncirus trifoliata and their primer sequences

基因功能归类 Category of gene function	基因名称 Gene name	序列ID Sequence ID	引物序列Primer sequence（5′-3′）
基因功能归类 Category of gene function	基因名称 Gene name	序列ID Sequence ID	正向引物 Forward primer	反向引物 Reverse primer
独脚金内酯相关 SLs-related	MAX1	Pt1g003810	TTGGGATACCTGTATGGA	GAGGGAATGCTTCTGTTT
	MAX2	Pt2g005380	ACCAACTGTCCGAAACTGA	AACGAGCCTACGACACCC
	IPA1	Pt3g040320	GGCGGAGATAACAAGCAC	CACCAGAACGGGAGAAAA
生长素相关 IAA-related	ARF5	Pt5g002170	TGGCACATCTTCGAGTAA	ATCGCAGAGCATAGTTCA
	ATHB8	Pt1g004770	ATGAGGGATGGTCTATGTT	AGAAGTATTGCTGGAGGC
	PIN1	Pt1g010310	AGAGGTGCCAGGTTGCTT	TTGATGGGCGTGGAGTTG
赤霉素相关 GA-related	SLR1	Pt6g004680	TTACGGTGGCGGTAGTGG	TTGTGGGTACGCAGCAGA
赤霉素相关 GA-related	DELLA	Pt2g018850	GCGGGTTTGGGTTTAGAT	GCCGATGATAGGCTCAGATA
木质部相关 Xylem-related	KNAT1	Pt5g011400	AGCCAAGATCATCTCCCA	TCGTAATAAGCCTCCATAA
	STM	Pt2g013810	CACTTCCCTCGCCTCTTG	CTGTCGGGCTTCCTTTGG
	CAD	Pt8g003710	GGGTTCTCGTTGGTTGTT	CTCCAGAGGGTGATTTGC
韧皮部相关 Phloem-related	NEN1/2	Pt3g006130	ATTGACTCGTTGGCATTG	CACATCTCCCACCTGTTC
韧皮部相关 Phloem-related	NEN4	Pt7g000360	AGTTCGGAGCAATCGTAG	ATATTATGCCCTGCCCAC
形成层相关 Cambium-related	OPS	Pt6g013590	GACACTGGGTCAGCGAGAT	TAGCAGGAGACACCTTAGCA
	WOX4	Pt5g004640	CTCACGCTTGGCTGTAAACG	GTTCGCATTCCTCCCCTGTA
	WOX14	Pt7g020570	TCAGTGCCAGACAGAGGT	AGGGAGGTTGCGTAAGTG
	KNAT6	Pt4g006750	CCGATGAGTTACTGTCTGCT	CTCCTACCTTCTGGCAAT
	BES1	Pt5g003460	AGGGCTCAGGGAAACTAT	ATGATGGAACTGGGCTTG

Fig. 2 Effect of different concentrations of GR24 on anatomical structure of the base stem of Poncirus trifoliata seedlings Red：Lignification；Green：Non lignification. Pi：Pith；Xy：Xylem；Ca：Cambium；Ph：Phloem

Fig. 3 Effect of different concentrations of GR24 on the thickness of xylem，cambium and phloem of the base stem of Poncirus trifoliata seedlings

Fig. 4 Effect of different concentrations of GR24 on the thickness of secondary xylem cell wall in the stem of Poncirus trifoliata seedlings A-D：TEM of xylem fibers in the base stem of plant with GR24 treatments；E-H：Higher magniffcation views of the areas in boxes in A-D

Fig. 5 Effect of different concentrations of GR24 on the thickness of secondary xylem cell wall，lignin contents，cellulose content，hemicellulose content in the stem of Poncirus trifoliata seedlings

Fig. 6 Effect of 20 μmol · L-1 GR24 on endogenous hormone contents in Poncirus trifoliata seedlings stems t-test，asterisk（*）means signiffcant difference between GR24 treatment and control at P ≤ 0.05. The same below

Fig. 7 Effect of 20 μmol · L-1 GR24 on the transcript levels of hormone-related genes in Poncirus trifoliata seedlings stems

Fig. 8 Effect of 20 μmol · L-1 GR24 on the transcript levels of secondary growth-related gened in Poncirus trifoliata seedlings stems

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Effect of Exogenous Strigolactones on the Secondary Growth of Poncirus trifoliata Seedlings Stem

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