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Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1823-1832.doi: 10.16420/j.issn.0513-353x.2023-0468

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles     Next Articles

Genetic Diversity Analysis and Core Collection Construction of Magnolia biondii Germplasm

QIN Zilu, XU Zhengkang, DAI Xiaogang, CHEN Yingnan*()   

  1. State Key Laboratory of Tree Genetics and Breeding,Co-Innovation Center for Sustainable Forestry in Southern China,Key Laboratory of Tree Genetics and Biotechnology of Educational Department of China,Key Laboratory of Tree Genetics and Silvicultural Sciences of Jiangsu Province,Nanjing Forestry University,Nanjing 210037,China
  • Received:2024-01-15 Revised:2024-04-22 Online:2024-08-25 Published:2024-08-21
  • Contact: CHEN Yingnan

Abstract:

In this study,genome ploidy levels of 917 Magnolia biondii germplasms collected from Nanzhao Country,Hennan Province were analyzed by using the flow cytometry. Genetic diversity and genetic structure of the Magnolia biondii germplasms were investigated by using SSR markers. In addition,simulated annealing algorithm was used to construct a core collection for the M. biondii Pamp. germplasms based on the maximizing genetic diversity(SAGD)and allele maximization(SANA),respectively. The results showed that all the investigated M. biondii Pamp. germplasms were diploids. A total of 57 alleles(Na)were detected by 10 SSR primers,and the average of effective allele number(Ne)was 2.667. The Shannon’s information index(I),the observed heterozygosity(Ho),the expected heterozygosity(He)and the polymorphism information content(PIC)were 1.124,0.422,0.586 and 0.547,respectively,indicating a high genetic diversity in the germplasm resources of M. biondii Pamp. Both genetic structure and cluster analysis analyses indicated that the 917 M. biondii Pamp. germplasms could be divided into four subgroups,and there were obvious genetic exchanges among different subgroups. The genetic parameters of core collection obtained by SAGD were higher than those of SANA,therefore 183 (20% of the original collection)germplasms were selected through the SAGD method to construct the core collection of M. biondii Pamp. The retention rate of NaNeIHo and He was 94.74%,105.40%,103.38%,104.27% and 104.61%,respectively. The principal coordinate analysis(PCoA)showed that the core collection constructed by this study was uniformly distributed within the initial collection and had a good representation. The constructed core collection will provide a scientific basis for conservation and utilization of M. biondii Pamp. germplasm resources.

Key words: Magnolia biondii, SSR, genetic diversity, genetic structure, core collection