Functional Study of AP2/ERF in Response to Black Rot at Broccoli Seedling Stage

doi:10.16420/j.issn.0513-353x.2023-0469

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (11): 2523-2539.doi: 10.16420/j.issn.0513-353x.2023-0469

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Functional Study of AP2/ERF in Response to Black Rot at Broccoli Seedling Stage

TANG Zheng^*(), CHEN Sique, XU Qian, ZHONG Weijie, LIU Qing, ZHU Shiyang

Key Laboratory of Crop Breeding in South Zhejiang，Key Laboratory of Wenzhou Endemic Crop Biotechnology Innovation，Wenzhou Vocational College of Science & Technology/Wenzhou Academy of Agricultural Sciences，Wenzhou，Zhejiang 325006，China

Received:2024-06-27 Revised:2024-08-20 Online:2024-12-12 Published:2024-11-25
Contact: TANG Zheng

Abstract

Abstract:

To explore the response mechanism of black rot and identify disease-resistance related genes through RNA-Seq analysis of broccoli（Brassica oleracea L. var. italica）leaves infected by black rot pathogen. Gene Ontology（GO）analysis showed that up-regulated differentially expressed genes（DEGs）were significantly enriched in stress response and phytohormone signaling pathways after 3，5，and 7 days infected by pathogens compared to uninoculated. At these three time points，the up-regulated genes in overlapped DEGs encoded up to 32 AP2/ERF transcription factors. Their encoded protein sequence ranged from 132 to 344 amino acids in length，and its promoter contained a large number of cis-elements in response to abscisic acid，auxin，jasmonic acid，gibberellin and salicylic acid（SA）. SA treatment significantly enhanced the resistance of broccoli to black rot，and strongly induced the expression of AP2/ERF and defense-related genes. BolC1t04894H encodes an EAR type transcriptional suppressor ERF4，which is localized in the nucleus. Transient expression of broccoli ERF4 resulted in sensitivity of tobacco leaves to Xcc treatment. Electrophoretic Mobility Shift Assay（EMSA）result indicated that ERF4 could bind the GCC-box sequence in the promoter of WRKY33. Subsequently，double luciferase reporter system experiments showed that ERF4 inhibited WRKY33 expression. Taken together，the phytohormone signalling pathway are activated in broccoli after black rot pathogen infection，and SA strongly induces the AP2/ERF expression，thereby regulating a series of defense-related genes transcription to response black rot.

Key words: broccoli, AP2/ERF transcription factor, black rot, salicylic acid, disease resistance

TANG Zheng, CHEN Sique, XU Qian, ZHONG Weijie, LIU Qing, ZHU Shiyang. Functional Study of AP2/ERF in Response to Black Rot at Broccoli Seedling Stage[J]. Acta Horticulturae Sinica, 2024, 51(11): 2523-2539.

Figures/Tables 13

References 24

[1]	Bostock R M. 2005. Signal crosstalk and induced resistance:straddling the line between cost and benefit. Annual Review of Phytopathology, 43 (1):545-580.
[2]	Catinot J, Huang J B, Huang P Y, Tseng M Y, Chen Y L, Gu S Y, Lo W S, Wang L C, Chen Y R, Zimmerli L. 2015. ETHYLENE RESPONSE FACTOR 96 positively regulates Arabidopsis resistance to necrotrophic pathogens by direct binding to GCC elements of jasmonate- and ethylene-responsive defence genes. Plant,Cell & Environment, 38 (12):2721-2734.
[3]	Cui Bo, Hao Ping’an, Liang Fang, Zhang Yan,Wang Ximeng,Li Junlin,Jiang Suhua,Xu Shenping. 2020. Cloning and expression analysis of AP2/ERF family gene from Phalaenopsis under low temperature. Acta Horticulturae Sinica, 47 (1):85-97. (in Chinese)
	崔波, 郝平安, 梁芳, 张燕, 王喜蒙, 李俊霖, 蒋素华, 许申平. 2020. 蝴蝶兰AP2/ERF家族基因的克隆及在低温下表达特性分析. 园艺学报, 47 (1):85-97. doi: 10.16420/j.issn.0513-353x.2019-0091
[4]	Gerit B, Tino U, Uhrig J F, Yvonne P, Gust A A, Dierk S, Justin L. 2009. Flg 22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. Proceedings of the National Academy of Sciences of the United States of America, 106 (7):8067-8072.
[5]	Gu Y Q, Mary C W, Chakravarthy S. 2002. Tomato transcription factors Pti4,Pti5,and Pti 6 activate defense responses when expressed in Arabidopsis. The Plant Cell, 14 (4):817-831.
[6]	Han Fengqing, Li Zhansheng, Liu Yumei, Fang Zhiyuan, Yang Limei, Zhuang Mu, Zhang Yangyong, Lü Honghao, Wang Yong. 2019. Disease regularity and comprehensive control of broccoli black rot. China Vegetables,(11):98-101. (in Chinese)
	韩风庆, 李占省, 刘玉梅, 方智远, 杨丽梅, 庄木, 张扬勇, 吕红豪, 王勇. 2019. 青花菜黑腐病发病规律及综合防治措施. 中国蔬菜,(11):98-101.
[7]	Han Rui,Zhong Xionghui,Chen Denghui,Cui Jian,Yue Xiangqing,Xie Jianming,Kang Jungen. 2023. Cloning and functional analysis of BobHLH34 gene in cabbage that interacts with XopR from Xanthomonas. Acta Horticulturae Sinica, 50 (2):319-330. (in Chinese)
	韩睿, 钟雄辉, 陈登辉, 崔建, 乐祥庆, 颉建明, 康俊根. 2023. 黑腐病菌效应因子XopR的甘蓝靶标基因BobHLH34的克隆及功能分析. 园艺学报, 50 (2):319-330. doi: 10.16420/j.issn.0513-353x.2021-1274
[8]	Jinwook J, So Y W, Seok C S, Kim H. 2007. The barley ERF-type transcription factor HvRAF confers enhanced pathogen resistance and salt tolerance in Arabidopsis. Planta, 225 (3):575-588. pmid: 16937017
[9]	Jisha V, Dampanaboina L, Vadassery J, Mithofer A, Kappara S, Ramanan R. 2015. Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice. PLoS ONE, 10 (6):e0127831.
[10]	Lai Danni, Cai Xiang, Chen Donghong, Han Zhigang, Wu Lingshang, Si Jinping, Li Cong. 2022. Identification and expression analysis of southern blight disease responding miR171b and miR171b's target DcGRAS gene family in Dendrobium catenatum. Journal of Agricultural Biotechnology, 30 (10):1869-1883. (in Chinese)
	赖丹妮, 蔡翔, 陈东红, 韩之刚, 吴令上, 斯金平, 李聪. 2022. 铁皮石斛白绢病响应miR171b及miR171b靶标DcGRAS基因家族的鉴定及表达分析. 农业生物技术学报, 30 (10):1869-1883.
[11]	Lee J H, Hong J P, Oh S K, Lee S, Choi D, Kim W T. 2004. The ethylene-responsive factor like protein 1(CaERFLP1)of hot pepper(Capsicum annuum L.)interacts in vitro with both GCC and DRE/CRT sequences with different binding affinities:possible biological roles of CaERFLP1 in response to pathogen infection and high salinity conditions in transgenic tobacco plants. Plant Molecular Biology, 55 (1):61-81.
[12]	Li Jidong, Ni Jing, Ye Xia, Chen Peng, Tan Bin, Zhang Mengyang, Zheng Xianbo, Zhang Yu, Feng Jiancan. 2020. Genomic identification of jujube AP2/ERF transcription factors and their expression pattern during jujube witches’broom pathogenesis process. Acta Horticulturae Sinica, 47 (8):1463-1474. (in Chinese)
	李继东, 倪静, 叶霞, 陈鹏, 谭彬, 张梦洋, 郑先波, 张玉, 冯建灿. 2020. 枣AP2/ERF转录因子鉴定及其响应枣疯病植原体表达分析. 园艺学报, 47 (8):1463-1474. doi: 10.16420/j.issn.0513-353x.2019-0965
[13]	Liu D, Chen X, Liu J, Ye J, Guo Z. 2012. The rice ERF transcription factor OsERF 922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance. Journal of Experimental Botany, 63 (10):3899-3911.
[14]	Marta B L, Antonio M, Roberto S. 2002. Constitutive expression of ETHYLENE-RESPONSE-FACTOR1 in Arabidopsis confers resistance to several necrotrophic fungi. The Plant Journal, 29 (1):23-32.
[15]	McGrath K C, Dombrecht B, Manners J M, Schenk P M, Edgar C I, Maclean D J, Scheible W R, Udvardi M K, Kazan K. 2005. Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant Physiology, 139 (2):949-959. doi: 10.1104/pp.105.068544 pmid: 16183832
[16]	Xu Zhixuan, Ren Zhonghai. 2020. Re-identification of tomato AP2/ERF transcription factor superfamily and phenotypic analysis of the overexpressing SlERF.D.3lines. Acta Horticulturae Sinica, 47 (4):653-664. (in Chinese)
	徐志璇, 任仲海. 2020. 番茄AP2/ERF 超家族重鉴定及过表达SlERF.D.3株系表型分析. 园艺学报, 47 (4):653-664.
[17]	Tezuka D, Kawamata A, Kato H, Saburi W, Mori H, Imai R. 2019. The rice ethylene response factor OsERF83 positively regulates disease resistance to Magnaporthe oryzae. Plant Physiology Biochemistry, 135:263-271.
[18]	Xie W, Ding C, Hu H, Dong G, Zhang G, Qian Q, Ren D. 2022. Molecular events of rice AP2/ERF transcription factors. International Journal of Molecular Sciences, 23 (19):12013.
[19]	Yao Xingwei, Niu Guobao, Shan Xiaozheng, Liu Lili, Wen Zhenghua, Jiang Hanming, Zhang Xiaoli. 2018. Identification of resistance to black rot in cauliflower seedling stage. Heilongjiang Agricultural Sciences,(8):39-41. (in Chinese)
	姚星伟, 牛国保, 单晓政, 刘莉莉, 文正华, 江汉民, 张小丽. 2018. 花椰菜苗期黑腐病抗病性鉴定. 黑龙江农业科学,(8):39-41.
[20]	Yao Yurong, Hou Jianfei, Hao Yongjuan, Ben Haiyan, Wang Wanli. 2020. Differential expression analysis of transcriptome in cauliflower cultivars resistant to and susceptible to black rot at seedling stage. Shandong Agricultural Sciences, 52 (2):1-6. (in Chinese)
	姚玉荣, 霍建飞, 郝永娟, 贲海燕, 王万立. 2020. 花椰菜苗期黑腐病抗、感品种转录组差异表达分析. 山东农业科学, 52 (2):1-6.
[21]	Zhang G Y, Chen M, Li L C, Zhao S Y. 2009. Overexpression of the soybean GmERF3 gene,an AP2/ERF type transcription factor for increased tolerances to salt,drought,and diseases in transgenic tobacco. Journal of Experimental Botany, 60 (13):3899-3911.
[22]	Zhang X L, Liu Y M, Fang Z Y, Li Z S, Yang L M, Zhuang M, Zhang Y Y, Lv H H. 2016. Comparative transcriptome analysis between broccoli(Brassica oleracea var. italica)and wild cabbage(Brassica macrocarpa Guss.) in response to Plasmodiophora brassicae during different infection stages. Frontiers in Plant Science, 7:1929.
[23]	Zhang X L, Zhang Z J, Chen J, Chen Q, Wang X C, Huang R F. 2005. Expressing TERF1 in tobacco enhances drought tolerance and abscisic acid sensitivity during seedling development. Planta, 222 (3):494-501.
[24]	Zuo K J, Qin J, Zhao J Y, Ling H, Zhang L D, Cao Y F, Tang K X. 2007. Over-expression GbERF2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes. Gene, 391 (1-2):80-90.

目的	引物名称		引物序列（5′-3′）
Purpose	Primer name		Primer sequence
转录水平检测	BolC9t55823H		F：GGCGAAGGGGAAGCATTAC；R：TCTCCACGGAAAGTTCAGCA
Transcriptional level detection		BolC8t48739H		F：ACGCAACACTTAATTTTCCGGA；R：CTTCAGCCATCCCTTCCTCT
		BolC7t43635H		F：GCCGTCAAAGGGAAAGCATT；R：CACGCATCCTAAAAGCAGCT
		BolC7t42203H		F：GCAGCACCGTCGAATCATC；R：ACTACGATCCATCACCACAGA
		BolC6t38421H		F：AGTGTCCGGGGAGAAGAATC；R：TCGGAGAAGTCATCAAACCCT
		BolC5t33888H		F：GGCGACTGATGGGATTGTTC；R：TCGTAGAGACCTGCATCACC
		BolC3t17033H		F：CACCCTTGACTGCGATTCTC；R：GGCGACCTAAAAGCTGACG
		BolC2t10891H		F：GGGAAGCATTACAGAGGGGT；R：CACGCATCCTAAAAGCAGCT
		BolC1t04894H		F：CTTGAGCTTAGTCTTGGCGG；R：CTCCCACAAGCCATCTTCGT
		BolC1t04025H		F：CGGTATTAGGGTTTGGCTCG；R：GAACACCCATCCTCGTAGCT
		BolC1t01080H		F：TAGAGTTTGGTTGGGGACGT；R：ACCGTTCTCCTCCGCTTC
		BolC1t00081H		F：CCACACATGAGGCACGAGTA；R：TCTTGATACTCCTCTGCTTTTGG
		PAL2		F：TAACAATCGGACAAGTCGC；R：AGAGGTCGCTCCAAACCC
		CHS		F：ACTACTACTTCCGCATCACC；R：GGGACTTCAACCACCACTA
		CHI		F：GCTAACGCCTATCCGAGCTT；R：TGTACACATGGGAACTCTGGT
		WRKY33		F：CTGTGTTCAATGCCAGCCTT；R：TTGCTTCAGGTTCACTCCCA
		Actin		F：CCAGAGGTCTTGTTCCAGCCATC；R：GTTCCACCACTGAGCACAATGTTAC
亚细胞定位和凝胶迁移 Subcellar location and EMSA	ERF4-pENTR		F：GCAGGCTCCGCGGCCATGGTTAAGATGGGTTTGAA； R：AGCTGGGTCGGCGCGGGCCTGTTCCAACGGAGGAG
双荧光素酶报告系统试验 Dual-luciferase reporter	ERF4-62SK		F：TAGAACTAGTGGATCCCCCATGGTTAAGATGGGTTTGAA； R：TATCGAATTCCTGCAGCCCTCAGGCCTGTTCCAACGGAGGAG
system		WRKY33pro-LUC		F：AGCTGGGTCGGCGCGGGCCTGTTCCAACGGAGGAG； R：TAGAACTAGTGGATCCCCCACATAAAAAAATTGAAGCTT

目的	引物名称		引物序列（5′-3′）
Purpose	Primer name		Primer sequence
转录水平检测	BolC9t55823H		F：GGCGAAGGGGAAGCATTAC；R：TCTCCACGGAAAGTTCAGCA
Transcriptional level detection		BolC8t48739H		F：ACGCAACACTTAATTTTCCGGA；R：CTTCAGCCATCCCTTCCTCT
		BolC7t43635H		F：GCCGTCAAAGGGAAAGCATT；R：CACGCATCCTAAAAGCAGCT
		BolC7t42203H		F：GCAGCACCGTCGAATCATC；R：ACTACGATCCATCACCACAGA
		BolC6t38421H		F：AGTGTCCGGGGAGAAGAATC；R：TCGGAGAAGTCATCAAACCCT
		BolC5t33888H		F：GGCGACTGATGGGATTGTTC；R：TCGTAGAGACCTGCATCACC
		BolC3t17033H		F：CACCCTTGACTGCGATTCTC；R：GGCGACCTAAAAGCTGACG
		BolC2t10891H		F：GGGAAGCATTACAGAGGGGT；R：CACGCATCCTAAAAGCAGCT
		BolC1t04894H		F：CTTGAGCTTAGTCTTGGCGG；R：CTCCCACAAGCCATCTTCGT
		BolC1t04025H		F：CGGTATTAGGGTTTGGCTCG；R：GAACACCCATCCTCGTAGCT
		BolC1t01080H		F：TAGAGTTTGGTTGGGGACGT；R：ACCGTTCTCCTCCGCTTC
		BolC1t00081H		F：CCACACATGAGGCACGAGTA；R：TCTTGATACTCCTCTGCTTTTGG
		PAL2		F：TAACAATCGGACAAGTCGC；R：AGAGGTCGCTCCAAACCC
		CHS		F：ACTACTACTTCCGCATCACC；R：GGGACTTCAACCACCACTA
		CHI		F：GCTAACGCCTATCCGAGCTT；R：TGTACACATGGGAACTCTGGT
		WRKY33		F：CTGTGTTCAATGCCAGCCTT；R：TTGCTTCAGGTTCACTCCCA
		Actin		F：CCAGAGGTCTTGTTCCAGCCATC；R：GTTCCACCACTGAGCACAATGTTAC
亚细胞定位和凝胶迁移 Subcellar location and EMSA	ERF4-pENTR		F：GCAGGCTCCGCGGCCATGGTTAAGATGGGTTTGAA； R：AGCTGGGTCGGCGCGGGCCTGTTCCAACGGAGGAG
双荧光素酶报告系统试验 Dual-luciferase reporter	ERF4-62SK		F：TAGAACTAGTGGATCCCCCATGGTTAAGATGGGTTTGAA； R：TATCGAATTCCTGCAGCCCTCAGGCCTGTTCCAACGGAGGAG
system		WRKY33pro-LUC		F：AGCTGGGTCGGCGCGGGCCTGTTCCAACGGAGGAG； R：TAGAACTAGTGGATCCCCCACATAAAAAAATTGAAGCTT

比较组	上调	下调
Comparison group	Up regulated	Down regulated
0 d vs. 1 d	蛋白泛素化 Protein ubiquitination	DNA复制起始 DNA replication initiation
	光呼吸 Photorespiration	蛋白折叠 Protein refolding
	昼夜节律 Circadian rhythm	叶绿体 Chloroplast
	还原戊糖磷酸循环 Reductive pentose phosphate cycle
0 d vs. 3 d	伤害应答 Response to wounding	翻译 Translation
	盐胁迫应答 Response to salt stress	光合作用 Photosynthesis
	高温应答 Response to heat	叶绿体 Chloroplast
	氧化胁迫应答 Response to oxidative stress	钙离子结合 Calcium ion binding
	几丁质酶应答 Response to chitin	核糖体结构成分 Structural constituent of ribosome
	Karrikin应答 Response to karrikin	rRNA结合 rRNA binding
	茉莉酸应答 Response to jasmonic acid
	脱落酸应答 Response to abscisic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
0 d vs. 5 d	伤害应答 Response to wounding	光合作用 Photosynthesis
	盐胁迫应答 Response to salt stress	生长调节 Regulation of growth
	钙离子应答 Response to calcium ion	生长素激活信号通路 Auxin-activated signaling pathway
	氧化胁迫应答 Response to oxidative stress	叶绿体 Chloroplast
	几丁质酶应答 Response to chitin	叶绿素结合 Chlorophyll binding
	Karrikin应答 Response to karrikin
	茉莉酸应答 Response to jasmonic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
	防御应答 Defense response
0 d vs. 7 d	伤害应答 Response to wounding	光和作用 Photosynthesis
	盐胁迫应答 Response to salt stress	细胞壁组织 Cell wall organization
	氧化胁迫应答 Response to oxidative stress	碳水化合物代谢 Carbohydrate metabolic process
	茉莉酸应答 Response to jasmonic acid	叶绿体 Chloroplast
	几丁质酶应答 Response to chitin
	Karrikin应答 Response to karrikin
	水杨酸应答 Response to salicylic acid
	脱落酸应答 Response to abscisic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
	防御应答 Defense response

比较组	上调	下调
Comparison group	Up regulated	Down regulated
0 d vs. 1 d	蛋白泛素化 Protein ubiquitination	DNA复制起始 DNA replication initiation
	光呼吸 Photorespiration	蛋白折叠 Protein refolding
	昼夜节律 Circadian rhythm	叶绿体 Chloroplast
	还原戊糖磷酸循环 Reductive pentose phosphate cycle
0 d vs. 3 d	伤害应答 Response to wounding	翻译 Translation
	盐胁迫应答 Response to salt stress	光合作用 Photosynthesis
	高温应答 Response to heat	叶绿体 Chloroplast
	氧化胁迫应答 Response to oxidative stress	钙离子结合 Calcium ion binding
	几丁质酶应答 Response to chitin	核糖体结构成分 Structural constituent of ribosome
	Karrikin应答 Response to karrikin	rRNA结合 rRNA binding
	茉莉酸应答 Response to jasmonic acid
	脱落酸应答 Response to abscisic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
0 d vs. 5 d	伤害应答 Response to wounding	光合作用 Photosynthesis
	盐胁迫应答 Response to salt stress	生长调节 Regulation of growth
	钙离子应答 Response to calcium ion	生长素激活信号通路 Auxin-activated signaling pathway
	氧化胁迫应答 Response to oxidative stress	叶绿体 Chloroplast
	几丁质酶应答 Response to chitin	叶绿素结合 Chlorophyll binding
	Karrikin应答 Response to karrikin
	茉莉酸应答 Response to jasmonic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
	防御应答 Defense response
0 d vs. 7 d	伤害应答 Response to wounding	光和作用 Photosynthesis
	盐胁迫应答 Response to salt stress	细胞壁组织 Cell wall organization
	氧化胁迫应答 Response to oxidative stress	碳水化合物代谢 Carbohydrate metabolic process
	茉莉酸应答 Response to jasmonic acid	叶绿体 Chloroplast
	几丁质酶应答 Response to chitin
	Karrikin应答 Response to karrikin
	水杨酸应答 Response to salicylic acid
	脱落酸应答 Response to abscisic acid
	乙烯激活信号通路 Ethylene-activated signaling pathway
	防御应答 Defense response

基因号 Gene ID	位置 Position	氨基酸数 Amino acid	定位预测 Predicted location	拟南芥同源基因（ID）
基因号 Gene ID	位置 Position	氨基酸数 Amino acid	定位预测 Predicted location	Arabidopsis thaliana homologous gene
BolC9t56886H	Chr9：37 383 176 ~ 37 383 838	220	细胞核 Nucleus	ERF105（AT5G51190）
BolC9t55823H	Chr9：23 228 516 ~ 23 229 238	240	细胞核 Nucleus	ERF2（AT5G47220）
BolC8t51758H	Chr8：44 860 010 ~ 44 860 951	313	细胞核 Nucleus	ERF053（AT2G20880）
BolC8t48739H	Chr8：24 675 340 ~ 24 676 173	277	细胞核 Nucleus	ERF054（AT4G28140）
BolC8t48489H	Chr8：22 668 610 ~ 22 669 377	255	细胞核 Nucleus	ERF1A（AT4G17500）
BolC8t48488H	Chr8：22 653 145 ~ 22 654 017	290	细胞核 Nucleus	ERF6（AT4G17490）
BolC7t43994H	Chr7：41 081 592 ~ 41 082 626	344	细胞核 Nucleus	ARF14（AT3G25730）
BolC7t43635H	Chr7：38 015 941 ~ 38 016 609	222	细胞核 Nucleus	ERF2（AT5G47220）
BolC7t42203H	Chr7：24 871 566 ~ 24 872 072	168	细胞核 Nucleus	ERF11（AT1G28370）
BolC7t41924H	Chr7：21 967 071 ~ 21 967 469	132	细胞核 Nucleus	ERF095（AT3G23220）
BolC7t41923H	Chr7：21 965 156 ~ 21 965 572	138	细胞核 Nucleus	ERF098（AT3G23230）
BolC6t38421H	Chr6：32 042 058 ~ 32 042 651	197	细胞质，细胞核Cytoplasm，nucleus	ERF018（AT1G74930）
BolC5t33888H	Chr5：46 627 650 ~ 46 628 303	217	细胞核 Nucleus	ERF4（AT3G15210）
BolC5t29217H	Chr5： 2 216 228 ~ 2 216 962	244	细胞核 Nucleus	ERF094（AT1G06160）
BolC4t28631H	Chr4：64 415 607 ~ 64 416 320	237	细胞核 Nucleus	ERF13（AT2G44840）
BolC4t23118H	Chr4：11 617 647 ~ 11 618 985	216	细胞核 Nucleus	ERF112（AT2G33710）
BolC3t20871H	Chr3：67 338 370 ~ 67 339 152	260	细胞核 Nucleus	ERF109（AT4G34410）
BolC3t19761H	Chr3：57 780 467 ~ 57 780 985	172	细胞核 Nucleus	ERF11（AT1G28370）
BolC3t17033H	Chr3：28 276 839 ~ 28 277 453	204	细胞核 Nucleus	ERF4（AT3G15210）
BolC3t14461H	Chr3：10 357 105 ~ 10 357 806	233	细胞核 Nucleus	ERF15（AT2G31230）
BolC3t13677H	Chr3： 5 792 930 ~ 5 793 562	210	细胞质，细胞核Cytoplasm，nucleus	ERF016（AT5G21960）
BolC2t10891H	Chr2：48 942 393 ~ 48 943 094	233	细胞核 Nucleus	ERF2（AT5G47220）
BolC2t10520H	Chr2：44 830 922 ~ 44 831 503	193	细胞核 Nucleus	ERF9（AT5G44210）
BolC2t09547H	Chr2：30 078 233 ~ 30 079 165	310	细胞核 Nucleus	RAP2-4（AT1G78080）
BolC2t06548H	Chr2： 3 356 340 ~ 3 358 302	254	细胞核 Nucleus	ERF113（AT5G13330）
BolC2t06200H	Chr2： 1 544 843 ~ 1 545 811	322	细胞核 Nucleus	DREB2A（AT5G05410）
BolC1t04894H	Chr1：44 469 287 ~ 44 469 943	218	细胞核 Nucleus	ERF4（AT3G15210）
BolC1t04027H	Chr1：36 457 782 ~ 36 459 026	212	细胞核 Nucleus	ERF098（AT3G23230）
BolC1t04025H	Chr1：36 421 156 ~ 36 421 788	210	细胞核 Nucleus	ERF1B（AT3G23240）
BolC1t01080H	Chr1： 6 205 090 ~ 6 205 785	231	细胞核 Nucleus	ERF1A（AT4G17500）
BolC1t01079H	Chr1： 6 195 373 ~ 6 196 152	259	细胞核 Nucleus	ERF6（AT4G17490）
BolC1t00081H	Chr1： 423 978 ~ 424 808	276	细胞核 Nucleus	ERF060（AT4G39780）

Functional Study of AP2/ERF in Response to Black Rot at Broccoli Seedling Stage

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