Research of Jasmonic Acid Antagonize Ethylene to Delay the Flower Senescence of Oncidium hybridum

doi:10.16420/j.issn.0513-353x.2022-0732

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (8): 1769-1778.doi: 10.16420/j.issn.0513-353x.2022-0732

• Cultivation Physiology & Biochemistry • Previous Articles Next Articles

Research of Jasmonic Acid Antagonize Ethylene to Delay the Flower Senescence of Oncidium hybridum

OUYANG Xuanyue¹^,², ZENG Sixian¹^,², YU Yunyi¹^,³, WANG Qin¹^,³, LI Xinyue¹^,³, YUE Yuechong¹^,³, YU Rangcai¹^,²^,^**(), FAN Yanping¹^,³^,^**()

¹ Flower Research Center，South China Agricultural University，Guangzhou 510642，China
² College of Life Science，South China Agricultural University，Guangzhou 510642，China
³ College of Forestry and Landscape Architecture，South China Agricultural University，Guangzhou 510642，China

Received:2023-01-11 Revised:2023-06-20 Online:2023-08-25 Published:2023-08-23
Contact: YU Rangcai, FAN Yanping

Abstract

Abstract:

In this study，in order to explore the role of ethylene and jasmonic acid in the senescence of Oncidium hybridum ‘Honey Angle’ flowers，endogenous ethylene release rate，jasmonic acid content and senescence after anther cap shedding was investigated. In additon，senescence，endogenous ethylene release and the expression of OhACO1 and OhACO2（ethylene synthesis key genes）after methyl jasmonate and its inhibitors acetylsalicylic acid treatment were also observed. The results showed that anther cap shedding treatment could accelerate senescence of‘Honey Angle’flowers. The endogenous ethylene release rate had reached 168.23 nL · g^-1 · h^-1after 1 d of anther cap shedding treatment while the control had not yet released. Meanwhile，the endogenous jasmonic acid（JA）content in both treatment at 1 d was significantly higher than that at 0 d，but the content in the anther cap shedding treatment was 57.86% lower than that in the control. Moreover，exogenous methyl jasmonate treatment could significantly inhibited the senescence of flowers and reduced the expression of OhACO1 and OhACO2，while its inhibitors could promoted senescence and increased the expression of OhACO1 and OhACO2. These results indicated that JA could affect ethylene release by inhibiting ethylene synthesis，thereby delaying the senescence induced by anther cap shedding of O. hybridum‘Honey Angle’flowers.

Key words: Oncidium hybridum, cut flower, senescence, anther cap shedding, ethylene, jasmonic acid

OUYANG Xuanyue, ZENG Sixian, YU Yunyi, WANG Qin, LI Xinyue, YUE Yuechong, YU Rangcai, FAN Yanping. Research of Jasmonic Acid Antagonize Ethylene to Delay the Flower Senescence of Oncidium hybridum[J]. Acta Horticulturae Sinica, 2023, 50(8): 1769-1778.

Figures/Tables 6

Fig. 1 Effect of anther cap removal on flower lip senescence of Oncidium hybridum‘Honey Angle’

Fig. 2 Effect of anther cap removal on flower lip shrinking area of Oncidium hybridum‘Honey Angle’ * represents significant difference at 0.05 level. The same below.

Fig. 3 Effect of anther cap removal on ethylene release rate，water content and jasmonic acid content in flowers of Oncidium hybridum‘Honey Angle’

Fig. 4 Effect of methyl jasmonate（MeJA）and acetylsalicylic acid（ASA）treatment on the flower lip senescencen of Oncidium hybridum‘Honey Angle’induced by anther cap removal

Fig. 5 Effect of methyl jasmonate（MeJA）and acetylsalicylic acid（ASA）on flower lip shrinking area，water content and ethylene release rate in Oncidium hybridum‘Honey Angle’induced by anther cap removal

Fig. 6 Effect of methyl jasmonate（MeJA）and acetylsalicylic acid（ASA）on expression of key genes ACO1 and ACO2 in ethylene biosynthesis of Oncidium hybridum‘Honey Angle’

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Research of Jasmonic Acid Antagonize Ethylene to Delay the Flower Senescence of Oncidium hybridum

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