Endophytic Fungi and the Relations to the Fruit Drop of Citrus

doi:10.16420/j.issn.0513-353x.2022-1000

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (12): 2713-2722.doi: 10.16420/j.issn.0513-353x.2022-1000

• Plant Protection • Previous Articles Next Articles

Endophytic Fungi and the Relations to the Fruit Drop of Citrus

CUI Xuejing¹, ZHANG Jianing¹, CHEN Jianghua¹, JIA Jichun³, LIN Yang¹, FU Yanping¹^,²^,^**()

¹ Key Laboratory of Plant Pathology of Hubei Province，College of Plant Science & Technology，Huazhong Agricultural University，Wuhan 430070，China
² National R & D Center for Citrus Postharvest Technology，Wuhan 430070，China
³ College of Plant Protection，Shanxi Agricultural University，Taigu，Shanxi 030801，China

Received:2023-02-16 Revised:2023-09-14 Online:2023-12-25 Published:2023-12-29
Contact: FU Yanping

Abstract

Abstract:

In order to explore the relation of endophytic fungi and fruit drop，the diversity of fungi in the dropping and healthy fruits of Newhall navel orange during the second fruit drop stage was studied by Illumina MiSeq sequencing technology. It was found that there were significant increases in the richness，evenness of the fungal community in the dropping fruits compared to the healthy fruits，and the composition structure was also different between them. Twenty-three endophytic fungal strains were isolated successfully from the dropping fruits and classified based on ITS sequences. Through artificial inoculation，some endophytic fungal strains could cause yellowing of the young citrus fruits and increase the content of abscisic acid and ethylene release in the fruits. The results suggest that the change of the endophytic fungal communities and the resulting change in the ABA and ETH might be involved in the second drop of citrus fruit.

Key words: citrus, fruit drop, endophytic fungi, fungal community diversity, Illumina MiSeq, abscisic acid, ethylene

CUI Xuejing, ZHANG Jianing, CHEN Jianghua, JIA Jichun, LIN Yang, FU Yanping. Endophytic Fungi and the Relations to the Fruit Drop of Citrus[J]. Acta Horticulturae Sinica, 2023, 50(12): 2713-2722.

Figures/Tables 8

Fig. 1 The dropping（right） and healthy（left） fruits of Newhall navel orange

Table 1 Sequencing data preprocessing statistics and quality analysis of Newhall Navel Orange

果实样品 Sample	原始序列条数 Raw PE	有效序列条数 Clean reads		Q30/%
健康1 Healthy 1	88 653	85 423	231	99.37	96.36
健康2 Healthy 2	95 273	87 349	251	98.93	91.68
健康3 Healthy 3	85 932	82 303	239	99.12	95.78
黄化即将脱落1 Dropping 1	89 633	86 210	240	99.32	96.18
黄化即将脱落2 Dropping 2	93 149	89 388	226	99.24	95.96
黄化即将脱落3 Dropping 3	96 317	92 087	235	99.31	95.61

Fig. 2 ITS sequencing rarefaction curve of Newhall Navel Orange

Fig. 3 The α-diversity of fungal community in the dropping and healthy fruits of Newhall Navel Orange

Fig. 4 PCoA analysis of fungal community in the dropping and healthy fruits of Newhall Navel Orange based on the Weighted Unifrac distance

Fig. 5 The fungal community composition of fungal community in the dropping and healthy fruits of Newhall Navel Orange at the genus level

Fig. 6 Influence of fungal strains to Nawhall Navel Orange fruits

Fig. 7 Abscisic acid content（A）and ethylene released（B）by citrus fruits 50 d post flower at 11 d post inoculation of fungal strains HF means healthy fruits and DF means dropping fruits. NHE-3：Alternaria sp.;NHE-4: Mucor sp.;NHE-6：Arthrinium sp.;NHE-10：Aspergillus sp.;NHE-12：Cladosporium sp.;NHE-16 and NHE-22：Fusarium sp. PDA：Negative control. One-way ANOVA（P < 0.05）.

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Endophytic Fungi and the Relations to the Fruit Drop of Citrus

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