Studies on Function and Mechanism of CsNAC2 Transcription Factor in Resistance to Green Mold in Citrus

doi:10.16420/j.issn.0513-353x.2022-1208

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (12): 2701-2712.doi: 10.16420/j.issn.0513-353x.2022-1208

• Plant Protection • Previous Articles Next Articles

Studies on Function and Mechanism of CsNAC2 Transcription Factor in Resistance to Green Mold in Citrus

CHEN Qi¹(), LI Ting¹, CHEN Jialin¹, CHEN Ou¹, WANG Wenjun¹^,², YAO Shixiang¹^,², ZENG Kaifang¹^,²^,³^,^*()

¹ College of Food Science，Southwest University，Chongqing 400715，China
² Research Center of Food Storage & Logistics，Southwest University，Chongqing 400715，China
³ National Citrus Engineering Technology Research Center，Chongqing 400712，China

Received:2023-01-18 Revised:2023-05-08 Online:2023-12-25 Published:2023-12-29
Contact: ZENG Kaifang

Abstract

Abstract:

In order to investigate the effect of citrus transcription factor CsNAC2 on resistance to green mold，the coding sequence of CsNAC2 was cloned using‘Jincheng 447'as study material. Bioinformatics analysis，yeast one-hybrid assay，RNA-Seq，EMSA and qRT-PCR were employed to explore the regulatory function of CsNAC2 on fruit green mold resistance. The results showed that the coding sequence of CsNAC2 is 909 bp，encoding 302 amino acids，and the molecular weight of the protein is 34.40 kD. CsNAC2 possesses the structural characteristics of the NAC family and contains conserved NAC domain in the N terminal. Transient overexpression of CsNAC2 on citrus fruit could effectively reduce the incidence of green mold in fruit，with a considerable decrease in incidence and spot diameter. Transcriptomic analysis showed that CsNAC2 can participate in several metabolic pathways related to disease resistance in fruits. Further studies on lignin synthesis pathway，amino acid and nucleotide sugar metabolism pathway showed that CsNAC2 can bind the CsLAC7 and CsEP3 gene promoters in these two pathways，respectively. Transient overexpression of CsNAC2 increased the expression of CsLAC7 and CsEP3 genes. The comprehensive results indicated that CsNAC2 may activates the expression of CsLAC7 and CsEP3 genes to enhance the resistance to green mold in citrus fruits.

Key words: citrus, green mold, transcription factor CsNAC2, disease resistance, molecular mechanism

CHEN Qi, LI Ting, CHEN Jialin, CHEN Ou, WANG Wenjun, YAO Shixiang, ZENG Kaifang. Studies on Function and Mechanism of CsNAC2 Transcription Factor in Resistance to Green Mold in Citrus[J]. Acta Horticulturae Sinica, 2023, 50(12): 2701-2712.

Figures/Tables 9

References 33

[1]	Bai X X, Zhan G M, Tian S X, Peng H, Cui X Y, Islam M A, Goher F, Ma Y Z, Kang Z S, Xu Z S, Guo J. 2021. Transcription factor BZR2 activates chitinase Cht20.2 transcription to confer resistance to wheat stripe rust. Plant Physiology, 187 (4):2749-2762. doi: 10.1093/plphys/kiab383 pmid: 34618056
[2]	Berthet S, Thevenin J, Baratiny D, Caulet N D, Debeaujon I, Bidzinski P, Leple J C, Huis R, Hawkins S, Gomez L D, Lapierre C, Jouanin L. 2012. Role of plant laccases in lignin polymerization. Advances in Botanical Research, 61:145-172.
[3]	Cao X M, Wei C Y, Duan W Y, Gao Y, Kuang J F, Liu M C, Chen K S, Klee H, Zhang B. 2021. Transcriptional and epigenetic analysis reveals that NAC transcription factors regulate fruit flavor ester biosynthesis. Plant Journal, 106 (3):785. doi: 10.1111/tpj.v106.3 URL
[4]	Chang Y N, Yu R M, Feng J L, Chen H Z, Eri H, Gao G. 2020. NAC transcription factor involves in regulating bacterial wilt resistance in potato. Functional Plant Biology, 47 (10):925-936. doi: 10.1071/FP19331 pmid: 32454004
[5]	Chen Jiang-hua, Cui Xue-jing, Cheng Jia-sen, Lin Yang, Xie Jia-tao, Fu Yan-ping. 2019. Incidence dynamics of postharvest diseases of citrus in main producing areas of China. Journal of Huazhong Agricultural University, 38 (6):92-97. (in Chinese)
	陈江华, 崔雪婧, 程家森, 林杨, 谢甲涛, 付艳苹. 2019. 我国主要产区柑橘采后病害发生动态. 华中农业大学学报, 38 (6):92-97.
[6]	Chen Na,Jiang Jing,Cao Bi-hao,Lei Jian-jun,Chen Chang-ming. 2015. The latest progresses on plant NAC transcription factors function. Molecular Plant Breeding, 13 (6):1407-1414. (in Chinese)
	陈娜, 蒋晶, 曹必好, 雷建军, 陈长明. 2015. 植物NAC转录因子功能研究新进展. 分子植物育种, 13 (6):1407-1414.
[7]	Deng B, Wang W H, Deng L L, Yao S X, Ming J, Zeng K F. 2018. Comparative RNA-seq analysis of citrus fruit in response to infection with three major postharvest fungi. Postharvest Biology and Technology, 146:134-146. doi: 10.1016/j.postharvbio.2018.08.012 URL
[8]	Fu B L, Wang W Q, Liu X F, Duan X W, Allan A C, Donald G, Yin X R. 2021. An ethylene-hypersensitive methionine sulfoxide reductase regulated by NAC transcription factors increases methionine pool size and ethylene production during kiwifruit ripening. New Phytologist, 232 (1):237-251. doi: 10.1111/nph.v232.1 URL
[9]	Han Li-tao. 2022. Functional study of maize NAC tranxcription factor ZmNAC19[M. D. Dissertation]. Shenyang: Shenyang Agricultural University. (in Chinese)
	韩立涛. 2022. 玉米NAC类转录因子ZmNAC19的功能研究[硕士论文]. 沈阳: 沈阳农业大学.
[10]	Hu Jia-qi. 2020. Effects of fusion gene 4CL1-CCR on tobacco cell wall[Ph. D. Dissertation]. Beijing:Beijing Forestry University. (in Chinese)
	胡嘉祺. 2020. 融合基因4CL1-CCR对烟草细胞壁影响的研究[博士论文]. 北京: 北京林业大学.
[11]	Jamil W, Wu W, Ahmad M, Zhu Q G, Liu X F, Jin R, Yin X R. 2019. High-CO₂ /hypoxia-modulated NAC transcription factors involved in de-astringency of persimmon fruit. Scientia Horticulturae, 252:201-207. doi: 10.1016/j.scienta.2019.03.018 URL
[12]	Kalenahalli N Y, Kobir S, Udaykumar K, Ajjamada C K. 2017. Potato NAC43 and MYB 8 mediated transcriptional regulation of secondary cell wall biosynthesis to contain phytophthora infestans infection. Plant Molecular Biology Reporter, 35 (5):519-533. doi: 10.1007/s11105-017-1043-1 URL
[13]	Kong X M, Zhou Q, Zhou X, Wei B D, Ji S J. 2020. Transcription factor CaNAC 1 regulates low-temperature-induced phospholipid degradation in green bell pepper. Journal of Experimental Botany, 71 (3):1078-1091.
[14]	Kou X H, Zhao Y N, Wu C E, Jiang B L, Zhang Z, Jacob R R, He Y L, Xue Z H. 2018. SNAC4 and SNAC9 transcription factors show contrasting effects on tomato carotenoids biosynthesis and softening. Postharvest Biology and Technology, 144:9-19. doi: 10.1016/j.postharvbio.2018.05.008 URL
[15]	Liang Ke-hao. 2020. Functional analysis and validation of NAC transcription factors PwNAC30/PwNAC 31 in Picea wilsonii[M. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	梁珂豪. 2020. 青杄NAC转录因子PwNAC30/PwNAC31的功能研究与验证[硕士论文]. 北京: 北京林业大学.
[16]	Liang Pan,Li Yue-yan,Huang Shao-yun,Chen Liang-zhe,Yi Qing-ping. 2021. Research progress of postharvest storage and preservation technology of citrus fruits. Packaging Engineering, 42 (13):57-66. (in Chinese)
	梁攀, 李悦妍, 黄少云, 陈良哲, 易庆平. 2021. 柑橘类水果贮藏保鲜技术研究进展. 包装工程, 42 (13):57-66.
[17]	Liu Q, Yan S J, Huang W J, Yang J Y, Dong J F, Zhang S H, Zhao J L, Yang T F, Mao X X, Zhu X Y, Liu B. 2018. NAC transcription factor ONAC066 positively regulates disease resistance by suppressing the ABA signaling pathway in rice. Plant Molecular Biology, 98:289-302. doi: 10.1007/s11103-018-0768-z pmid: 30387038
[18]	Negi S, Tak H, Ganapathi T R. 2018. A banana NAC transcription factor(MusaSNAC1)impart drought tolerance by modulating stomatal closure and H₂O₂content. Plant Molecular Biology, 96:457-471. doi: 10.1007/s11103-018-0710-4
[19]	Niu F F, Wang B Y, Wu F F, Yan J L, Li L, Wang C, Wang Y Q, Yang B, Jiang Y Q. 2014. Canola(Brassica napus L.)NAC 103 transcription factor gene is a novel player inducing reactive oxygen species accumulation and cell death in plants. Biochemical and Biophysical Research Communications, 454 (1):30-35. doi: 10.1016/j.bbrc.2014.10.057 URL
[20]	Qin Juan, Yu Fan, Liu Lu, Zhu Ting-ting, Chen Wei, Chao Shi-feng, Yang Zhen-feng, Shi Li-yu. 2021. Cloning of peach PpNAC19 and its regulation on PpCCD 4 promoter activity. Journal of Nuclear Agricultural Sciences, 35 (6):1273-1280. (in Chinese) doi: 10.11869/j.issn.100-8551.2021.06.1273
	秦娟, 余凡, 刘璐, 朱婷婷, 陈伟, 曹士锋, 杨震峰, 施丽愉. 2021. 桃PpNAC19的克隆及其对PpCCD4启动子活性的调节分析. 核农学报, 35 (6):1273-1280. doi: 10.11869/j.issn.100-8551.2021.06.1273
[21]	Shan W, Chen J Y, Kuang J F, Lu W J. 2016. Banana fruit NAC transcription factor MaNAC 5 cooperates with MaWRKYS to enhance the expression of pathogenesis-related genes against Colletotrichum musae. Molecular Plant Pathology, 17 (3):330-338. doi: 10.1111/mpp.12281 pmid: 26033522
[22]	Song R R, Li J, Xie C J, Jian W, Yang X Y. 2020. An overview of the molecular genetics of plant resistance to the verticillium wilt pathogen Verticillium dahliae. International Journal of Molecular Sciences, 21 (3):1120. doi: 10.3390/ijms21031120 URL
[23]	Sun D Y, Zhang X G, Zhang Q Y, Ji X T, Jia Y, Wang H, Niu L X, Zhang Y L. 2019. Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor,LrNAC35,contributing to defence response against cucumber mosaic virus and tobacco mosaic virus. Molecular Plant Pathology, 20 (12):1662-1681. doi: 10.1111/mpp.v20.12 URL
[24]	Wang Fang, Sun Li-jiao, Zhao Xiao-yu, Wang Jie-wan, Song Xing-shun. 2019. Research progress on plant NAC transcription factors. Biotechnology Bulletin, 35 (4):88-93. (in Chinese)
	王芳, 孙立娇, 赵晓宇, 王婕婉, 宋兴舜. 2019. 植物NAC转录因子的研究进展. 生物技术通报, 35 (4):88-93. doi: 10.13560/j.cnki.biotech.bull.1985.2018-0905
[25]	Xu Q, Wang W Q, Zeng J K, Zhang J, Donald G, Li X, Yin X R, Chen K S. 2015. A NAC transcription factor,EjNAC1,affects lignification of loquat fruit by regulating lignin. Postharvest Biology and Technology, 102:25-31. doi: 10.1016/j.postharvbio.2015.02.002 URL
[26]	Yang H B, Zhu Z F, Zhang M F, Li X, Xu R W, Zhu F, Xu J, Deng X X, Cheng Y J. 2022. QTL analysis reveals reduction of fruit water loss by NAC 042 through regulation of cuticular wax synthesis in citrus fruit. Horticultural Plant Journal, 8 (6):737-746. doi: 10.1016/j.hpj.2022.10.009 URL
[27]	Zhang Xiao-meng. 2017. Identification and preliminary function analysis of nac transcription factors in cucumber and tomato[Ph. D. Dissertation]. Beijing:Chinese Academy of Agricultural Sciences. (in Chinese)
	张晓孟. 2017. 黄瓜番茄NAC转录因子鉴定及在逆境应答和果实发育中的功能初步分析[博士论文]. 北京: 中国农业科学院.
[28]	Zheng Lin,Wang Shuai,Liu Yunuo,Du Meixia,Peng Aihong,He Yongrui,Chen Shanchun,Zou Xiuping. 2022. Gene cloning and expression analysis of NAC gene in citrus in response to Hunaglongbing. Acta Horticulturae Sinica, 49 (7):1441-1457. (in Chinese)
	郑林, 王帅, 刘语诺, 杜美霞, 彭爱红, 何永睿, 陈善春, 邹修平. 2022. 柑橘响应黄龙病菌侵染的NAC基因的克隆及表达分析. 园艺学报, 49 (7):1441-1457. doi: 10.16420/j.issn.0513-353x.2021-0553 URL
[29]	Zhou Ya-han. 2017. Salicylic acid,pichia membranaefaciens and oligochitosan induced disease resistance of citrus fruit and the possible biological mechanisms involved[Ph. D. Dissertation]. Chongqing:Southwest University. (in Chinese)
	周雅涵. 2017. 水杨酸、膜醭毕赤酵母、壳寡糖诱导柑橘果实抗病性及其生物学机制研究[博士论文]. 重庆: 西南大学.
[30]	Zhou Yang-kai. 2019. The mechanism of transcription gactor NbNAC 1 regulates plants systemic resistance against virus invasion and preparation of its polyclonal antibody[M. D. Dissertation]. Yangzhou: Yangzhou University. (in Chinese)
	周杨开. 2019. 转录因子NbNAC1调控植物系统性抗性防御病毒入侵的机制及其蛋白抗体制备[硕士论文]. 扬州: 扬州大学.
[31]	Zhu F, Luo T, Liu C Y, Wang Y, Zheng L, Xiao X, Zhang M F, Yang H B, Yang W, Xu R W, Zeng Y L, Ye J L, Xu J, Xu J G, Larkin R M, Wang P W, Wen W W, Deng X X, Fernie A R, Cheng Y J. 2020. A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED 5 in Citrus reticulata. Journal of Experimental Botany, 71 (12):12.
[32]	Zhu Ziguo, Yin Qizhong, Zhang Qingtian, Han Zhen, Zhang Qian, Li Bo. 2020. DRL1,a NAC gene from Vitis vinifera‘Yatomo Rose',negatively regulates the drought tolerance. Acta Horticulturae Sinica, 47 (12):2290-2300. (in Chinese)
	朱自果, 阴启忠, 张庆田, 韩真, 张倩, 李勃. 2020. 欧洲葡萄‘粉红亚都蜜'NAC基因DRL1负向调节植物抗旱性. 园艺学报, 47 (12):2290-2300.
[33]	Zhun Feng-juan. 2005. Introduction to chitinase in plants. Chinese Wild Plant Resources,(5):46-47,61. (in Chinese)
	朱凤鹃. 2005. 谈谈植物中的几丁质酶. 中国野生植物资源,(5):46-47,61.

引物名称 Primer name	引物序列（5′-3′） Sequence
CsNAC2-For	ATGACGGCTGAGTTACAGTTACCA
CsNAC2-Rev	TTAAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-pGBKT7-For	AGGCCGAATTCCCGGGGATCCATGACGGCTGAGTTACAGTTACCA
CsNAC2-pGBKT7-Rev	CTAGTTATGCGGCCGCTGCAGTTAAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-pEAQ-For	CAAATTCGCGACCGGTATGACGGCTGAGTTACAGTTACCA
CsNAC2-pEAQ-Rev	AGTTAAAGGCCTCGAGAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-GST-For	GGTTCCGCGTGGATCCATGACGGCTGAGTTACAGTTACCA
CsNAC2-GST-Rev	AGTCACGATGCGGCCGCAAAGGGTTTCGGAAGGTACATGAA
CsLAC7-probe-For	AATTTTTCACGGCACTGCTTCAATTGTAATGCGTGTGTCAGTAG
CsLAC7-probe-Rev	TGCTGACAAGCTACTGACACACGCATTACAATTGAAGCAGTG
CsLAC7-mutant probe-For	AATTTTTAAAAGCACTAAAACAATTGTAATGAAAATGTCAGTAAAAAGTCAGCA
CsLAC7-mutant probe-Rev	TGCTGACTTTTTACTGACATTTTCATTACAATTGTTTTAGTGCTTTTAAAAATT
CsEP3-probe-For	TACTCGCTCGACAATAGATCCGTAGTCAACACGGAGGACAAGAA
CsEP3-probe-Rev	TTCTTGTCCTCCGTGTTGACTACGGATCTATTGTCGAGCGAGTA
CsEP3-mutant probe-For	TACTCGCTCGACAATAGATCAAAAGTCAAAAAAGAGGACAAGAA
CsEP3-mutant probe-Rev	TTCTTGTCCTCTTTTTTGACTTTTGATCTATTGTCGAGCGAGTA
CsLAC7-For	CCTGCAAAACACAGCGTTGA
CsLAC7-Rev	GTAGGGATCCCGTCCTGAAA
CsEP3-For	TGTGTCAAAACTCCGTTGCC
CsEP3-Rev	AGCACAATGAGACGGAGAACT
Action-For	ATCTGCTGGAAGGTGCTGAG
Action-Rev	CCAAGCAGCATGAAGATCAA

引物名称 Primer name	引物序列（5′-3′） Sequence
CsNAC2-For	ATGACGGCTGAGTTACAGTTACCA
CsNAC2-Rev	TTAAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-pGBKT7-For	AGGCCGAATTCCCGGGGATCCATGACGGCTGAGTTACAGTTACCA
CsNAC2-pGBKT7-Rev	CTAGTTATGCGGCCGCTGCAGTTAAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-pEAQ-For	CAAATTCGCGACCGGTATGACGGCTGAGTTACAGTTACCA
CsNAC2-pEAQ-Rev	AGTTAAAGGCCTCGAGAAAGGGTTTCGGAAGGTACATGAA
CsNAC2-GST-For	GGTTCCGCGTGGATCCATGACGGCTGAGTTACAGTTACCA
CsNAC2-GST-Rev	AGTCACGATGCGGCCGCAAAGGGTTTCGGAAGGTACATGAA
CsLAC7-probe-For	AATTTTTCACGGCACTGCTTCAATTGTAATGCGTGTGTCAGTAG
CsLAC7-probe-Rev	TGCTGACAAGCTACTGACACACGCATTACAATTGAAGCAGTG
CsLAC7-mutant probe-For	AATTTTTAAAAGCACTAAAACAATTGTAATGAAAATGTCAGTAAAAAGTCAGCA
CsLAC7-mutant probe-Rev	TGCTGACTTTTTACTGACATTTTCATTACAATTGTTTTAGTGCTTTTAAAAATT
CsEP3-probe-For	TACTCGCTCGACAATAGATCCGTAGTCAACACGGAGGACAAGAA
CsEP3-probe-Rev	TTCTTGTCCTCCGTGTTGACTACGGATCTATTGTCGAGCGAGTA
CsEP3-mutant probe-For	TACTCGCTCGACAATAGATCAAAAGTCAAAAAAGAGGACAAGAA
CsEP3-mutant probe-Rev	TTCTTGTCCTCTTTTTTGACTTTTGATCTATTGTCGAGCGAGTA
CsLAC7-For	CCTGCAAAACACAGCGTTGA
CsLAC7-Rev	GTAGGGATCCCGTCCTGAAA
CsEP3-For	TGTGTCAAAACTCCGTTGCC
CsEP3-Rev	AGCACAATGAGACGGAGAACT
Action-For	ATCTGCTGGAAGGTGCTGAG
Action-Rev	CCAAGCAGCATGAAGATCAA

途径Pathway	基因 Gene symbol	log₂(FC)	功能注释 Description	基因编号 Citrus sinensis ID
苯丙烷生物合成 Phenylpropanoid biosynthesis	COMT1	1.07	咖啡酸3甲基转移酶 Caffeic acid 3-O-Methyltransferase-like	Cs5g13580
	COMT	1.29	咖啡酸3甲基转移酶 Caffeic acid 3-O-Methyltransferase-like	Cs5g18050
	LAC7	1.18	漆酶 Laccase-7-like	Cs6g07400
类胡萝卜素生物合成 Carotenoid biosynthesis	NCED1	1.18	9-顺式环氧类胡萝卜素双加氧酶 9-cis-Epoxycarotenoid dioxygenase	Cs2g03270
类胡萝卜素生物合成 Carotenoid biosynthesis	NCED3	1.42	9-顺式环氧类胡萝卜素双加氧酶 9-cis-Epoxycarotenoid dioxygenase	Cs5g14370
氨基酸和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	EP3	1.38	几丁质酶 Chitinase	Cs5g21870
角质素、软木脂和蜡质生物合成 Cutin, suberine and wax biosynthesis	CER1	1.22	乙醛脱羧酶 Aldehyde decarbonylase	Cs1g02750
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism	CAS2	1.29	半胱胺酸合成酶 Cysteine synthase	orange1.1t00386
谷胱甘肽代谢 Glutathione metabolism	HSP26-A	1.06	谷胱甘肽S转移酶 Glutathione S-transferase	Cs7g15760
植物激素信号转导 Plant hormone signal transduction	IAA29	1.25	植物激素响应蛋白IAA Auxin-responsive protein IAA	Cs4g18240

途径Pathway	基因 Gene symbol	log₂(FC)	功能注释 Description	基因编号 Citrus sinensis ID
苯丙烷生物合成 Phenylpropanoid biosynthesis	COMT1	1.07	咖啡酸3甲基转移酶 Caffeic acid 3-O-Methyltransferase-like	Cs5g13580
	COMT	1.29	咖啡酸3甲基转移酶 Caffeic acid 3-O-Methyltransferase-like	Cs5g18050
	LAC7	1.18	漆酶 Laccase-7-like	Cs6g07400
类胡萝卜素生物合成 Carotenoid biosynthesis	NCED1	1.18	9-顺式环氧类胡萝卜素双加氧酶 9-cis-Epoxycarotenoid dioxygenase	Cs2g03270
类胡萝卜素生物合成 Carotenoid biosynthesis	NCED3	1.42	9-顺式环氧类胡萝卜素双加氧酶 9-cis-Epoxycarotenoid dioxygenase	Cs5g14370
氨基酸和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	EP3	1.38	几丁质酶 Chitinase	Cs5g21870
角质素、软木脂和蜡质生物合成 Cutin, suberine and wax biosynthesis	CER1	1.22	乙醛脱羧酶 Aldehyde decarbonylase	Cs1g02750
半胱氨酸和蛋氨酸代谢 Cysteine and methionine metabolism	CAS2	1.29	半胱胺酸合成酶 Cysteine synthase	orange1.1t00386
谷胱甘肽代谢 Glutathione metabolism	HSP26-A	1.06	谷胱甘肽S转移酶 Glutathione S-transferase	Cs7g15760
植物激素信号转导 Plant hormone signal transduction	IAA29	1.25	植物激素响应蛋白IAA Auxin-responsive protein IAA	Cs4g18240

Studies on Function and Mechanism of CsNAC2 Transcription Factor in Resistance to Green Mold in Citrus

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 33

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	JIN Tian, XU Yuemei, KUANG Guanling, and LIU Guidong, . Effect of Boron Deficiency on the Root Growth and Mitochondrial Function of Trifoliate Orange Seedlings [J]. Acta Horticulturae Sinica, 2024, 51(1): 121-132.
[2]	LI Suping, LI Wei, HAN Leng, and HUANG Jianguo, . Bacillus velezensis HY19 Volatile Organic Compounds as a Preservative in Postharvest Citrus Fruit Management [J]. Acta Horticulturae Sinica, 2024, 51(1): 162-174.
[3]	CHEN Min, WU Tianli, Lü Yuanda, JIANG Bo, YAN Huaxue, LI Juan, ZHONG Yun. Analysis on Growth，Physiology and Fruit Quality of‘Hongjiang’Orange Grafted with Different Rootstocks Under Container Culture [J]. Acta Horticulturae Sinica, 2023, 50(7): 1547-1562.
[4]	DENG Chengfeng, LI Suping, ZHANG Ruixuan, HAN Leng, LI Yong. Control Effect of Lysobacter enzymogenes LE16 on Rot Disease in Post-harvest Citrus Fruit Caused by Penicillium digitatum and P. italicum [J]. Acta Horticulturae Sinica, 2023, 50(7): 1574-1586.
[5]	ZHANG Lehuan, ZOU Changyu, WANG Zhaohao, YANG Wen, ZOU Xiuping, HE Yongrui, CHEN Shanchun, LONG Qin. Cloning and Expression Analysis of CsAOS1-2 in Responding to Citrus Canker Disease [J]. Acta Horticulturae Sinica, 2023, 50(6): 1355-1367.
[6]	GUO Jing, LIAO Manyu, JIN Yan, MA Xiaochuan, ZHANG Fen, LU Xiaopeng, DENG Ziniu, SHENG Ling. Functional Analysis of Transcription Factor CsbHLH3 in Regulating Citric Acid Metabolism of Citrus Fruit [J]. Acta Horticulturae Sinica, 2023, 50(5): 947-958.
[7]	ZHOU Ping, GUO Rui, YAN Shaobin, JIN Guang. Molecular Mechanism Study of Exogenous Sorbitol Effects on Sugar Metabolism in Peach Leaves and Fruits [J]. Acta Horticulturae Sinica, 2023, 50(5): 959-971.
[8]	LÜ Ruoya, LI Yun, ZHENG Yongqin, DENG Xiaoling, ZHENG Zheng. The Distribution Pattern of Candidatus Liberibacter asiaticus in Fruit Pith [J]. Acta Horticulturae Sinica, 2023, 50(5): 1110-1117.
[9]	WANG Ping, SHENG Ling, YANG Jinpeng, ZHOU Linglei, JIN Yan, LUO Xuzhao, MA Xianfeng, DENG Ziniu. Evaluation of Resistance to Citrus Canker Disease in Hybrid Progeny of Red Pomelo and American Citron [J]. Acta Horticulturae Sinica, 2023, 50(4): 765-777.
[10]	ZOU Yunqian, LUO Qujuan, ZHANG Jin, XU Rangwei, CHENG Yunjiang. Coating Containing Shellac，Rosin Significantly Improves Commercial Value of Satsuma Mandarins and Lane Late Navel Orange During Shelf Life [J]. Acta Horticulturae Sinica, 2023, 50(4): 853-863.
[11]	LAI Hengxin, LI Wenguang, PENG Liangzhi, HE Yizhong, ZHU Panpan, YANG Wanyun, LING Lili, FU Xingzheng, CHUN Changpin, CAO Li. Quality Changes of On-tree Storage Fruit of Orah Mandarin（Citrus reticulata Blanco）During Spring and Summer Seasons [J]. Acta Horticulturae Sinica, 2023, 50(3): 485-494.
[12]	LIU Yunuo, CAO Ya, WANG Shuai, DU Meixia, ZHENG Lin, CHEN Shanchun, ZOU Xiuping. Expression Analysis of CsMYB41 and CsMYB63 Genes in Response to Citrus Canker [J]. Acta Horticulturae Sinica, 2023, 50(3): 495-507.
[13]	LEI Jianjun, ZHU Zhangsheng, CHEN Changming, CHEN Guoju, CAO Bihao, ZHENG Jie, WU Hao, XIAO Yanhui, QIU Zhengkun, YAN Shuangshuang. Research Progress on Pepper Main Red Pigments and Their Molecular Mechanisms of Biosynthesis [J]. Acta Horticulturae Sinica, 2023, 50(3): 669-684.
[14]	YE Zimao, SHEN Wanxia, LIU Mengyu, WANG Tong, ZHANG Xiaonan, YU Xin, LIU Xiaofeng, ZHAO Xiaochun. Effect of R2R3-MYB Transcription Factor CitMYB21 on Flavonoids Biosynthesis in Citrus [J]. Acta Horticulturae Sinica, 2023, 50(2): 250-264.
[15]	LIANG Shengmin, ZHANG Fei, WU Qiangsheng. Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Trifoliate Orange Seedlings by Regulating Root Polyamines [J]. Acta Horticulturae Sinica, 2023, 50(12): 2680-2688.