Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Trifoliate Orange Seedlings by Regulating Root Polyamines

doi:10.16420/j.issn.0513-353x.2022-1036

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (12): 2680-2688.doi: 10.16420/j.issn.0513-353x.2022-1036

• Plant Protection • Previous Articles Next Articles

Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Trifoliate Orange Seedlings by Regulating Root Polyamines

LIANG Shengmin¹, ZHANG Fei², WU Qiangsheng¹^,^*()

¹ College of Horticulture and Gardening，Yangtze University，Jingzhou，Hubei 434025，China
² College of Biology and Agricultural Resources，Huanggang Normal University，Huanggang，Hubei 438000，China

Received:2023-05-07 Revised:2023-09-06 Online:2023-12-25 Published:2023-12-29
Contact: WU Qiangsheng

Abstract

Abstract:

In order to clarify whether and how arbuscular mycorrhizal fungi regulate polyamines to enhance drought tolerance of citrus，trifoliate orange（Poncirus trifoliata）seedlings were inoculated with an arbuscular mycorrhizal fungus Rhizophagus intraradices，and the changes in growth，leaf water potential，reactive oxygen species levels，polyamines and polyamine precursors levels，and polyamine synthesis/cleavage enzyme activities were analyzed under well-watered and drought stress. The drought with nine weeks inhibited the colonization rate of R. intraradices in roots by 16.85%. Drought also significantly inhibited the growth and leaf water potential of trifoliate orange，while inoculation with R. intraradices considerably mitigated the inhibitive effect. Inoculation with R. intraradices significantly increased agmatine，S-adenosyl-L-methionine，putrescine，and cadaverine concentrations in roots under drought stress，along with the significant reduction in spermidine，spermine，L-arginine，L-ornithine，hydrogen peroxide，and superoxide anion radicals levels in roots. R. intraradices inoculation also distinctly elevated activities of various polyamine synthetases，such as arginine decarboxylase，ornithine decarboxylase，spermidine synthetase，spermine synthetase，and S-adenosyl methionine decarboxylase，and also increased activities of polyamine metabolism enzymes，such as diamine oxidase and polyamine oxidase. In addition，putrescine and cadaverine concentrations were significantly and negatively correlated with root superoxide anion radicals levels，while spermidine concentrations were positively correlated with root hydrogen peroxide and superoxide anion radicals levels. Therefore，it is concluded that arbuscular mycorrhizal fungi alter polyamine levels in roots of drought-stressed trifoliate orange to reduce reactive oxygen species burst.

Key words: citrus, drought tolerance, mycorrhiza, putrescine, reactive oxygen species

LIANG Shengmin, ZHANG Fei, WU Qiangsheng. Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Trifoliate Orange Seedlings by Regulating Root Polyamines[J]. Acta Horticulturae Sinica, 2023, 50(12): 2680-2688.

Figures/Tables 7

References 30

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处理 Treatment	干旱胁迫前 Pre-drougth stress			干旱胁迫后 Post-drought stress
处理 Treatment	侵染率/% Colonization	株高/cm Height	茎粗/mm Stem diameter	侵染率/% Colonization	株高/cm Height	茎粗/mm Stem diameter
-AMF + WW	0.00 ± 0.00 b	5.55 ± 0.91 c	1.91 ± 0.22 b	0.00 ± 0.00 c	13.58 ± 0.69 c	2.14 ± 0.22 b
+AMF + WW	54.07 ± 7.85 a	17.83 ± 3.13 a	2.78 ± 0.24 a	84.97 ± 7.24 a	27.91 ± 3.18 a	3.38 ± 0.24 a
-AMF + DS	0.00 ± 0.00 b	4.07 ± 1.11 c	1.89 ± 0.12 b	0.00 ± 0.00 c	11.11 ± 0.83 d	2.04 ± 0.12 b
+AMF + DS	55.05 ± 8.27 a	15.01 ± 1.37 b	2.73 ± 0.23 a	70.65 ± 3.45 b	24.99 ± 1.44 b	3.31 ± 0.23 a

处理 Treatment	干旱胁迫前 Pre-drougth stress			干旱胁迫后 Post-drought stress
处理 Treatment	侵染率/% Colonization	株高/cm Height	茎粗/mm Stem diameter	侵染率/% Colonization	株高/cm Height	茎粗/mm Stem diameter
-AMF + WW	0.00 ± 0.00 b	5.55 ± 0.91 c	1.91 ± 0.22 b	0.00 ± 0.00 c	13.58 ± 0.69 c	2.14 ± 0.22 b
+AMF + WW	54.07 ± 7.85 a	17.83 ± 3.13 a	2.78 ± 0.24 a	84.97 ± 7.24 a	27.91 ± 3.18 a	3.38 ± 0.24 a
-AMF + DS	0.00 ± 0.00 b	4.07 ± 1.11 c	1.89 ± 0.12 b	0.00 ± 0.00 c	11.11 ± 0.83 d	2.04 ± 0.12 b
+AMF + DS	55.05 ± 8.27 a	15.01 ± 1.37 b	2.73 ± 0.23 a	70.65 ± 3.45 b	24.99 ± 1.44 b	3.31 ± 0.23 a

处理 Treatment	生物量/（g · plant^-1）Biomass		根冠比 Root/shoot ratio	叶片水势/MPa Leaf water potential
处理 Treatment	地上部 Shoot	地下部 Root	根冠比 Root/shoot ratio	叶片水势/MPa Leaf water potential
-AMF + WW	0.86 ± 0.06 c	1.31 ± 0.12 c	1.54 ± 0.17 a	-0.43 ± 0.02 bc
+AMF + WW	2.07 ± 0.18 a	2.22 ± 0.29 a	1.07 ± 0.14 b	-0.36 ± 0.02 a
-AMF + DS	0.75 ± 0.07 c	1.04 ± 0.10 d	1.40 ± 0.17 a	-0.45 ± 0.03 c
+AMF + DS	1.83 ± 0.31 b	1.81 ± 0.24 b	0.99 ± 0.10 b	-0.40 ± 0.03 b

处理 Treatment	生物量/（g · plant^-1）Biomass		根冠比 Root/shoot ratio	叶片水势/MPa Leaf water potential
处理 Treatment	地上部 Shoot	地下部 Root	根冠比 Root/shoot ratio	叶片水势/MPa Leaf water potential
-AMF + WW	0.86 ± 0.06 c	1.31 ± 0.12 c	1.54 ± 0.17 a	-0.43 ± 0.02 bc
+AMF + WW	2.07 ± 0.18 a	2.22 ± 0.29 a	1.07 ± 0.14 b	-0.36 ± 0.02 a
-AMF + DS	0.75 ± 0.07 c	1.04 ± 0.10 d	1.40 ± 0.17 a	-0.45 ± 0.03 c
+AMF + DS	1.83 ± 0.31 b	1.81 ± 0.24 b	0.99 ± 0.10 b	-0.40 ± 0.03 b

处理 Treatment	L-精氨酸 L-Arg	L-鸟氨酸 L-Orn	胍基丁胺 Agm	S-腺苷-L-蛋氨酸 SAM
-AMF + WW	353.4 ± 37.0 d	7.73 ± 0.72 d	0.32 ± 0.02 d	1.01 ± 0.07 c
+AMF + WW	564.6 ± 31.8 a	20.60 ± 2.07 a	2.45 ± 0.11 a	3.18 ± 0.17 a
-AMF + DS	504.5 ± 17.8 b	16.71 ± 1.15 b	0.61 ± 0.05 c	1.16 ± 0.06 c
+AMF + DS	401.2 ± 18.5 c	10.13 ± 1.27 c	1.24 ± 0.10 b	1.79 ± 0.08 b

Arbuscular Mycorrhizal Fungi Improve Drought Tolerance of Trifoliate Orange Seedlings by Regulating Root Polyamines

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处理 Treatment	腐胺/ （nmol · g^-1 FW） Put	尸胺/ （nmol · g^-1 FW） Cad	亚精胺/ （pmol · g^-1 FW） Spd	精胺/ （pmol · g^-1 FW） Spm	（亚精胺 + 精胺）/腐胺（Spm + Spd）/Put
-AMF + WW	28.12 ± 2.09 d	0.41 ± 0.04 c	207.7 ± 5.7 a	46.51 ± 3.17 b	0.0090 ± 0.0004 a
+AMF + WW	56.30 ± 3.75 b	0.70 ± 0.09 a	164.0 ± 13.4 b	26.55 ± 4.74 d	0.0034 ± 0.0004 c
-AMF + DS	40.73 ± 2.58 c	0.56 ± 0.04 b	136.6 ± 12.1 c	55.58 ± 2.26 a	0.0047 ± 0.0004 b
+AMF + DS	62.00 ± 3.87 a	0.66 ± 0.06 a	98.9 ± 4.0 d	32.76 ± 3.58 c	0.0021 ± 0.0002 d

处理 Treatment	多胺合成酶/ (U · g^-1) Polyamine associated synthase					多胺分解酶/ (U · g^-1) Polyamine cleavage enzyme
处理 Treatment	精氨酸脱羧酶 ADC	鸟氨酸脱羧酶 ODC	亚精胺合成酶 SPDS	精胺合成酶 SPMS	S-腺苷蛋氨酸脱羧酶SAMDC	多胺氧化酶 PAO	二胺氧化酶 DAO
-AMF + WW	78.1 ± 5.9 c	160.7 ± 11.3 c	98.1 ± 12.9 c	569.5 ± 53.6 c	233.7 ± 24.9 c	298.4 ± 14.8 c	22.06 ± 2.20 b
+AMF + WW	96.4 ± 6.5 b	192.1 ± 8.3 b	126.6 ± 8.5 ab	644.0 ± 52.0 b	277.2 ± 37.7 ab	339.6 ± 12.5 b	26.25 ± 2.42 a
-AMF + DS	86.9 ± 3.4 bc	182.6 ± 10.5 b	124.6 ± 13.7 b	665.2 ± 34.6 b	251.1 ± 24.2 bc	313.9 ± 24.7 bc	22.05 ± 2.41 b
+AMF + DS	114.7 ± 9.2 a	209.1 ± 11.3 a	143.6 ± 10.4 a	745.2 ± 40.0 a	317.0 ± 30.1 a	367.9 ± 16.3 a	27.67 ± 0.90 a

多胺PA	精氨酸脱羧酶 ADC	鸟氨酸脱羧酶 ODC	亚精胺合成酶 SPDS	精胺合成酶 SPMS	S-腺苷蛋氨酸脱羧酶SAMDC	多胺氧化酶 PAO	二胺氧化酶 DAO	超氧阴离子Superoxide anion	H₂O₂
腐胺Put	0.86^**	0.80^**	0.78^**	0.71^**	0.71^**	0.84^**	0.74^**	-0.50^*	-0.16
尸胺Cad	0.64^**	0.66^**	0.78^**	0.47	0.54^*	0.65^**	0.55^*	-0.53^*	-0.19
精胺Spm	-0.79^**	-0.82^**	-0.82^**	-0.85^**	-0.64^**	-0.72^**	-0.52^*	-0.08	-0.43
亚精胺Spd	-0.62^*	-0.54^*	-0.4	-0.23	-0.46	-0.65^*	-0.71^**	0.88^**	0.71^**

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