Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (12): 2641-2652.doi: 10.16420/j.issn.0513-353x.2023-0046
• Cultivation Physiology & Biochemistry • Previous Articles Next Articles
LIU Xiaodong, WU Fang, MENG Chuan, MU Jingui, WANG Yuhai, WANG Mingqiu**()
Received:
2023-07-31
Revised:
2023-10-16
Online:
2023-12-25
Published:
2023-12-29
Contact:
WANG Mingqiu
LIU Xiaodong, WU Fang, MENG Chuan, MU Jingui, WANG Yuhai, WANG Mingqiu. The Culture Technique of Isolated Microspore in Tetraploid Chinese Cabbage[J]. Acta Horticulturae Sinica, 2023, 50(12): 2641-2652.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0046
编号 No. | 生育期/d Days | 叶球类型 Leafy head | 编号 No. | 生育期/d Days | 叶球类型 Leafy head |
---|---|---|---|---|---|
D20 | 55 ~ 60 | 花心 Spreading type | D522 | 55 ~ 65 | 叠抱 Overlapping type |
D21 | 55 ~ 60 | 合抱 Outward-curling type | D523 | 55 ~ 65 | 叠抱 Overlapping type |
D22 | 55 ~ 60 | 花心 Spreading type | D524 | 55 ~ 65 | 叠抱 Overlapping type |
D23 | 55 ~ 60 | 花心 Spreading type | D525 | 55 ~ 65 | 合抱Outward-curling type |
D24 | 55 ~ 60 | 叠抱 Overlapping type | D526 | 55 ~ 65 | 花心 Spreading type |
D520 | 55 ~ 65 | 花心 Spreading type | D527 | 55 ~ 65 | 合抱Outward-curling type |
D521 | 55 ~ 65 | 花心 Spreading type | D528 | 55 ~ 65 | 合抱Outward-Curling Type |
Table 1 Information table of 14 early mature Chinese cabbage inbred lines
编号 No. | 生育期/d Days | 叶球类型 Leafy head | 编号 No. | 生育期/d Days | 叶球类型 Leafy head |
---|---|---|---|---|---|
D20 | 55 ~ 60 | 花心 Spreading type | D522 | 55 ~ 65 | 叠抱 Overlapping type |
D21 | 55 ~ 60 | 合抱 Outward-curling type | D523 | 55 ~ 65 | 叠抱 Overlapping type |
D22 | 55 ~ 60 | 花心 Spreading type | D524 | 55 ~ 65 | 叠抱 Overlapping type |
D23 | 55 ~ 60 | 花心 Spreading type | D525 | 55 ~ 65 | 合抱Outward-curling type |
D24 | 55 ~ 60 | 叠抱 Overlapping type | D526 | 55 ~ 65 | 花心 Spreading type |
D520 | 55 ~ 65 | 花心 Spreading type | D527 | 55 ~ 65 | 合抱Outward-curling type |
D521 | 55 ~ 65 | 花心 Spreading type | D528 | 55 ~ 65 | 合抱Outward-Curling Type |
NAA/ (mg · L-1) | IBA/ (mg · L-1) | 根长/cm Root length | 须根数量 Fibrous root | 生根率/% Rooting rate | 移栽成活率/% Transplantation survival rate |
---|---|---|---|---|---|
0 | 0 | — | — | — | — |
0.1 | 3.51 ± 0.83 de | 少 Few | 50.0 a | 36.7 b | |
0.1 | 0 | 3.15 ± 0.07 e | 较少 Fewer | 33.3 a | 10.0 a |
0.1 | 3.86 ± 0.06 de | 多 More | 90.0 ab | 90.0 c | |
0.2 | 4.59 ± 1.41 d | 多 More | 100.0 b | 90.0 c | |
0.3 | 8.26 ± 1.05 c | 多 More | 100.0 b | 100.0 c | |
0.4 | 10.19 ± 0.39 b | 少 Few | 100.0 b | 100.0 c | |
0.5 | 11.51 ± 1.42 a | 少 Few | 93.3 b | 93.3 c |
Table 2 Root regeneration of regenerated plants under different mass concentration of NAA and IBA
NAA/ (mg · L-1) | IBA/ (mg · L-1) | 根长/cm Root length | 须根数量 Fibrous root | 生根率/% Rooting rate | 移栽成活率/% Transplantation survival rate |
---|---|---|---|---|---|
0 | 0 | — | — | — | — |
0.1 | 3.51 ± 0.83 de | 少 Few | 50.0 a | 36.7 b | |
0.1 | 0 | 3.15 ± 0.07 e | 较少 Fewer | 33.3 a | 10.0 a |
0.1 | 3.86 ± 0.06 de | 多 More | 90.0 ab | 90.0 c | |
0.2 | 4.59 ± 1.41 d | 多 More | 100.0 b | 90.0 c | |
0.3 | 8.26 ± 1.05 c | 多 More | 100.0 b | 100.0 c | |
0.4 | 10.19 ± 0.39 b | 少 Few | 100.0 b | 100.0 c | |
0.5 | 11.51 ± 1.42 a | 少 Few | 93.3 b | 93.3 c |
Fig. 2 The best process of selecting flower bud in microspore culture of tetraploid chinese cabbage A:Schematic diagram of petal length(L1)and anther length(L);B:The bud L1/L between 0 and 0.5;C:The bud L1/L greater than 0.5;D:Late uninucleate stage;E:Binucleate stage.
Fig. 3 Plant regeneration during microspore culture A:Embryos regenerated from microspore;B:Embryogenic culture;C:Embryo regeneration;D:Rooting culture.
编号 No. | 鉴定植株数 Number of plant | 二倍体数 Number of diploid | 四倍体数 Number of autotetraploid | 二倍体率/% Diploid rate | 四倍体率/% Autotetraploid rate |
---|---|---|---|---|---|
D24 | 8 | 8 | 0 | 100.00 | 0 |
D522 | 18 | 16 | 2 | 88.89 | 11.11 |
D524 | 7 | 7 | 0 | 100.00 | 0 |
D528 | 6 | 6 | 0 | 100.00 | 0 |
合计Sum | 39 | 37 | 2 | 94.87 | 5.13 |
Table 3 Ploidy composition of microspore regenerated plants from tetraploid Chinese cabbage
编号 No. | 鉴定植株数 Number of plant | 二倍体数 Number of diploid | 四倍体数 Number of autotetraploid | 二倍体率/% Diploid rate | 四倍体率/% Autotetraploid rate |
---|---|---|---|---|---|
D24 | 8 | 8 | 0 | 100.00 | 0 |
D522 | 18 | 16 | 2 | 88.89 | 11.11 |
D524 | 7 | 7 | 0 | 100.00 | 0 |
D528 | 6 | 6 | 0 | 100.00 | 0 |
合计Sum | 39 | 37 | 2 | 94.87 | 5.13 |
小孢子再生植株 Microspore regenerated plants | 授粉品种Varieties | 小孢子再生植株 Microspore regenerated plants | 授粉品种Varieties | ||
---|---|---|---|---|---|
冀白4号(2x) Jibai 4 | 多维462(4x) Duowei 462 | 冀白4号(2x) Jibai 4 | 多维462(4x) Duowei 462 | ||
XD24-1(2x) | 3.19 | 0 | XD522-13(2x) | 4.14 | 0 |
XD24-2(2x) | 2.00 | 0 | XD522-14(2x) | 4.73 | 0 |
XD24-3(2x) | 1.63 | 0 | XD522-15(2x) | 1.05 | 0 |
XD24-4(2x) | 1.55 | 0 | XD522-16(2x) | 2.87 | 0 |
XD24-5(2x) | 1.97 | 0 | XD522-17(2x) | 1.56 | 0 |
XD24-6(2x) | 2.66 | 0 | XD522-18(2x) | 1.51 | 0 |
XD24-7(2x) | 2.48 | 0 | XD524-1(2x) | 1.04 | 0 |
XD24-8(2x) | 3.06 | 0 | XD524-2(2x) | 2.29 | 0 |
XD522-1(4x) | 0 | 1.15 | XD524-3(2x) | 0.99 | 0 |
XD522-2(4x) | 0 | 0.83 | XD524-4(2x) | 3.06 | 0 |
XD522-3(2x) | 5.01 | 0 | XD524-5(2x) | 2.68 | 0 |
XD522-4(2x) | 7.69 | 0 | XD524-6(2x) | 2.77 | 0 |
XD522-5(2x) | 3.23 | 0 | XD524-7(2x) | 3.64 | 0 |
XD522-6(2x) | 5.11 | 0 | XD528-1(2x) | 0.87 | 0 |
XD522-7(2x) | 2.24 | 0 | XD528-2(2x) | 2.96 | 0 |
XD522-8(2x) | 1.17 | 0 | XD528-3(2x) | 3.49 | 0 |
XD522-9(2x) | 4.38 | 0 | XD528-4(2x) | 1.98 | 0 |
XD522-10(2x) | 2.57 | 0 | XD528-5(2x) | 1.86 | 0 |
XD522-11(2x) | 2.36 | 0 | XD528-6(2x) | 3.59 | 0 |
XD522-12(2x) | 5.52 | 0 |
Table 4 Seed quantity of microspore regenerated plants hybridized with diploid‘Jibai 4'and tetraploid‘Duowei 462'respectively
小孢子再生植株 Microspore regenerated plants | 授粉品种Varieties | 小孢子再生植株 Microspore regenerated plants | 授粉品种Varieties | ||
---|---|---|---|---|---|
冀白4号(2x) Jibai 4 | 多维462(4x) Duowei 462 | 冀白4号(2x) Jibai 4 | 多维462(4x) Duowei 462 | ||
XD24-1(2x) | 3.19 | 0 | XD522-13(2x) | 4.14 | 0 |
XD24-2(2x) | 2.00 | 0 | XD522-14(2x) | 4.73 | 0 |
XD24-3(2x) | 1.63 | 0 | XD522-15(2x) | 1.05 | 0 |
XD24-4(2x) | 1.55 | 0 | XD522-16(2x) | 2.87 | 0 |
XD24-5(2x) | 1.97 | 0 | XD522-17(2x) | 1.56 | 0 |
XD24-6(2x) | 2.66 | 0 | XD522-18(2x) | 1.51 | 0 |
XD24-7(2x) | 2.48 | 0 | XD524-1(2x) | 1.04 | 0 |
XD24-8(2x) | 3.06 | 0 | XD524-2(2x) | 2.29 | 0 |
XD522-1(4x) | 0 | 1.15 | XD524-3(2x) | 0.99 | 0 |
XD522-2(4x) | 0 | 0.83 | XD524-4(2x) | 3.06 | 0 |
XD522-3(2x) | 5.01 | 0 | XD524-5(2x) | 2.68 | 0 |
XD522-4(2x) | 7.69 | 0 | XD524-6(2x) | 2.77 | 0 |
XD522-5(2x) | 3.23 | 0 | XD524-7(2x) | 3.64 | 0 |
XD522-6(2x) | 5.11 | 0 | XD528-1(2x) | 0.87 | 0 |
XD522-7(2x) | 2.24 | 0 | XD528-2(2x) | 2.96 | 0 |
XD522-8(2x) | 1.17 | 0 | XD528-3(2x) | 3.49 | 0 |
XD522-9(2x) | 4.38 | 0 | XD528-4(2x) | 1.98 | 0 |
XD522-10(2x) | 2.57 | 0 | XD528-5(2x) | 1.86 | 0 |
XD522-11(2x) | 2.36 | 0 | XD528-6(2x) | 3.59 | 0 |
XD522-12(2x) | 5.52 | 0 |
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