The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance

doi:10.16420/j.issn.0513-353x.2021-0025

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 319-331.doi: 10.16420/j.issn.0513-353x.2021-0025

• Research Papers • Previous Articles Next Articles

The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance

PENG Yi, LI Yuanhui, YANG Rui, ZHANG Ziyi, LI Yanan, HAN Yunhao, ZHAO Wenchao(), WANG Shaohui()

Beijing Key Laboratory for Agriculture Application and New Technique,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China

Received:2021-03-15 Revised:2021-04-19 Online:2022-02-25 Published:2022-02-28
Contact: ZHAO Wenchao,WANG Shaohui E-mail:zwcxy1985@163.com;wangshaohui@bua.edu.cn

Abstract

Abstract:

To study the drought resistance mechanism regulated by JA,using tomato variety‘Castlemart’jasmonic acid synthesis gene LoxD-overexpression transgenic plants（LoxD-oe）and LoxD mutant（spr8）and their corresponding wild type（WT）as material. Under the drought stress,the relative water content,soluble sugar,malondialdehyde content,stomatal aperture,H₂O₂ level in guard cells,and the expressions of the ABA signaling and related ion channel genes were determined. It was found that LoxD gene was induced by drought stress. The overexpression of LoxD gene gave rise to the increases of the relative water content and soluble sugar content and the decline of MDA production in leaves. Moreover,under drought stress,overexpressing LoxD gene induced the expressions of SnRK2-like,SLAC1,AtrbohD and CPK1,inhibited the expressions of ABI2 and KAT1,and activated the H₂O₂ signal in guard cells,which probably arrested the stomatal opening and then reduced the water loss. The results showed that LoxD gene participates in regulating drought resistance.

Key words: tomato, jasmonic acid, drought stress, LoxD gene, stomatal movement, H₂O₂ fluorescence signal

CLC Number:

S641.2

PENG Yi, LI Yuanhui, YANG Rui, ZHANG Ziyi, LI Yanan, HAN Yunhao, ZHAO Wenchao, WANG Shaohui. The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance[J]. Acta Horticulturae Sinica, 2022, 49(2): 319-331.

Figures/Tables 10

Table 1 Primer sequences for qRT-PCR

基因 Gene	LOC编号 LOC ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
LoxD	Solyc03g122340	TCCATAACTTAATTCCATCTC	TGATAGTGCTAACAACCT
LoxC	Solyc01g006540	GAGCTAACTAGGCCACCAATAG	CCGGAGTCATGAGAAAGAACA
AOS1	Solyc04g079730	CATCATCATCGTCATCAC	GAAGTAATCAAGTCTGTCTT
AOC	Solyc02g085730	CTATCTTCTGCCTTCCAA	TGTTAGTTGAATCTGTTGAG
SnRK2-like	Solyc01g108280	TGGGACCAACAGCCTTAATC	GGTCAGGATCACTCTCCAAATC
SLAC1 AtrbohD	Solyc08g079770.2 Solyc03g117980	GCAGAGGAGGTTCCATCTTTC TACTGTCCCACTAGCACTCTAT	GCAGAAGGGTTGAGACGAATAA GTGAAGGGCCAACACATTTC
CPK1	Solyc03g031670	GAACTCAACTGGGAACCCTTAT	TCCCTCTTCATATCCTCCCTATC
ABI2	Solyc03g121880	CAGACTGTATTGGGAGCTGAAA	CATGGATCCTCTCCAACAGATAAA
KAT1	Solyc02g070530	GACCAGATGAGGGAACAAAGAG	GCCACTTGAACTGGTACTACAT

Fig. 1 Transcriptional response of tomato JA synthesis genes under drought stress

Fig. 2 Effect of MeJA on drought resistance of wild-type （WT）tomato under drought stress

Fig. 3 Phenotype of wild-type（WT）, LoxD-oe,spr8 tomato plants under drought treatment

Fig. 4 Relative water content（A）,soluble sugar content（B）and malondialdehyde content（C）of WT, LoxD-oe and spr8 tomatoes under drought stress P < 0.05.

Fig. 5 Stomatal aperture of WT, LoxD-oe,spr8 leaves under drought stress

Table 2 Percentage statistics of stomata opening in the epidermis of tomato WT,LoxD-oe and spr8 under drought stress

处理 Treatment	番茄 Tomato	开放/% Opening
对照 Control	WT	83.19
	LoxD-oe	67.44
	spr8	84.21
干旱 Drought	WT	56.41
	LoxD-oe	24.63
	spr8	65.71

Fig. 6 Levels of H2O2 generation from guard cells of WT（spraying 50 μmol · L-1 MeJA）

Fig. 7 Levels of H2O2 generation from guard cells of WT,LoxD-oe,spr8（under drought stress）

Fig. 8 Transcription level of guard cell stomatal pathway genes under drought stress P < 0.05.

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The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance

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