Progress and Prospect of Genome Editing in Tomato

doi:10.16420/j.issn.0513-353x.2020-0976

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 221-232.doi: 10.16420/j.issn.0513-353x.2020-0976

• Reviews • Previous Articles Next Articles

Progress and Prospect of Genome Editing in Tomato

ZHANG Aiping¹, LIU Jiangna¹, YAN Jianjun², ZHANG Xiying¹, BAI Yunfeng²()

¹Institute of Agriculture Science,Sixth Dicision,Xinjiang Ptoduction and Construction Crop,Wujiaqu,Xinjiang 831300,China
²College of Agriculture,Shanxi Agricultural University,Taiyuan 030031,China

Received:2021-02-19 Revised:2021-08-06 Online:2022-01-25 Published:2022-01-24

Abstract

Abstract:

In this review,we reviewed the recent progresses of the genome editing technology and its application in tomato. We summarized successful examples in fruit storage tolerance and ripening regulation,nutrient composition and fruit color,male sterility and parthenocarpy,stress response,plant architecture,inflorescencetype and de novo domestication of wild tomato species. We further stated the technical innovations of the CRISPR/Cas-based genome editing,such as single-base editing,DNA-free editing and the improvement of homologous recombination efficiency. We finally showed the prospects and challenges in applying the genome editing technology in tomato genetic improvement and new variety breeding.

Key words: genome editing, tomato, utilization, prospect

CLC Number:

S641.2

ZHANG Aiping, LIU Jiangna, YAN Jianjun, ZHANG Xiying, BAI Yunfeng. Progress and Prospect of Genome Editing in Tomato[J]. Acta Horticulturae Sinica, 2022, 49(1): 221-232.

Figures/Tables 1

References 64

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性状 Trait	靶基因 Target gene	基因编辑技术 Genome editing technology	修复途径 Repair pathway	参考文献 Reference
耐贮性/成熟调控	Alc	CRISPR/Cas9	NHEJ,HR	Yu et al.,2017
Storage / ripening	RIN	CRISPR/Cas9	NHEJ	Ito et al.,2015
	SP5G	CRISPR/Cas9	NHEJ	Soyk et al.,2017b
	α-Man	CRISPR/Cas9	NHEJ	张圆圆等,2019
	lncRNA1459	CRISPR/Cas9	NHEJ	Li et al.,2018a
	ORRM4	CRISPR/Cas9	NHEJ	Yang et al.,2017
果实营养	GAD2/GAD3	CRISPR/Cas9	NHEJ	Nonaka et al.,2017
Fruit nutrition	GABA-T/SSADH	CRISPR/Cas9	NHEJ	Li et al.,2018b
	SGR1/LCY-E/Blc/LCY-B1/LCY-B2	CRISPR/Cas9	NHEJ	Li et al.,2018e
果色Fruit color	MYB12	CRISPR/Cas9	NHEJ	Deng et al.,2018
	PSY1/ CRTISO	CRISPR/Cas9	NHEJ,HR	Dahan-Meir et al.,2018
雄性不育	STR1	CRISPR/Cas9	NHEJ	Du et al.,2020
Male sterility	AP3	CRISPR/Cas9	NHEJ	刘玉琛等,2019
	MPK20	CRISPR/Cas9	NHEJ	Chen et al.,2018
单性结实	AGL6	CRISPR/Cas9	NHEJ	Klap et al.,2017
Parthenocarpy	IAA9	CRISPR/Cas9	NHEJ	Ueta et al.,2017
生物胁迫反应	Mlo1	CRISPR/Cas9	NHEJ	Nekrasov et al.,2017
Biotic stress reponse	JAZ 2	CRISPR/Cas9	NHEJ	Ortigosa et al.,2018
非生物胁迫反应	PROCERA	TALEN	NHEJ	Lor et al.,2014
Abiotic stress reponse	MAPK3	CRISPR/Cas9	NHEJ	Wang et al.,2017
	NPR1	CRISPR/Cas9	NHEJ	Li et al.,2019a
	CBF1	CRISPR/Cas9	NHEJ	Li et al.,2018c
株型Plant architecture	J2/EJ2/LIN	CRISPR/Cas9	NHEJ	Soyk et al.,2017a
	AGO7	CRISPR/Cas9	NHEJ	Brooks et al.,2014
	CLV3	CRISPR/Cas9	NHEJ	Rodríguez-Leal et al.,2017
株色Plant color	ANT1	TALEN,CRISPR/Cas9	HR	Cermak et al.,2015
花序Inflorescence	L1L4	ZFN	NHEJ	Hilioti et al.,2016
	RRA3a	CRISPR/Cas9	NHEJ	Xu et al.,2015
	BOP	CRISPR/Cas9	NHEJ	Xu et al.,2016

性状 Trait	靶基因 Target gene	基因编辑技术 Genome editing technology	修复途径 Repair pathway	参考文献 Reference
耐贮性/成熟调控	Alc	CRISPR/Cas9	NHEJ,HR	Yu et al.,2017
Storage / ripening	RIN	CRISPR/Cas9	NHEJ	Ito et al.,2015
	SP5G	CRISPR/Cas9	NHEJ	Soyk et al.,2017b
	α-Man	CRISPR/Cas9	NHEJ	张圆圆等,2019
	lncRNA1459	CRISPR/Cas9	NHEJ	Li et al.,2018a
	ORRM4	CRISPR/Cas9	NHEJ	Yang et al.,2017
果实营养	GAD2/GAD3	CRISPR/Cas9	NHEJ	Nonaka et al.,2017
Fruit nutrition	GABA-T/SSADH	CRISPR/Cas9	NHEJ	Li et al.,2018b
	SGR1/LCY-E/Blc/LCY-B1/LCY-B2	CRISPR/Cas9	NHEJ	Li et al.,2018e
果色Fruit color	MYB12	CRISPR/Cas9	NHEJ	Deng et al.,2018
	PSY1/ CRTISO	CRISPR/Cas9	NHEJ,HR	Dahan-Meir et al.,2018
雄性不育	STR1	CRISPR/Cas9	NHEJ	Du et al.,2020
Male sterility	AP3	CRISPR/Cas9	NHEJ	刘玉琛等,2019
	MPK20	CRISPR/Cas9	NHEJ	Chen et al.,2018
单性结实	AGL6	CRISPR/Cas9	NHEJ	Klap et al.,2017
Parthenocarpy	IAA9	CRISPR/Cas9	NHEJ	Ueta et al.,2017
生物胁迫反应	Mlo1	CRISPR/Cas9	NHEJ	Nekrasov et al.,2017
Biotic stress reponse	JAZ 2	CRISPR/Cas9	NHEJ	Ortigosa et al.,2018
非生物胁迫反应	PROCERA	TALEN	NHEJ	Lor et al.,2014
Abiotic stress reponse	MAPK3	CRISPR/Cas9	NHEJ	Wang et al.,2017
	NPR1	CRISPR/Cas9	NHEJ	Li et al.,2019a
	CBF1	CRISPR/Cas9	NHEJ	Li et al.,2018c
株型Plant architecture	J2/EJ2/LIN	CRISPR/Cas9	NHEJ	Soyk et al.,2017a
	AGO7	CRISPR/Cas9	NHEJ	Brooks et al.,2014
	CLV3	CRISPR/Cas9	NHEJ	Rodríguez-Leal et al.,2017
株色Plant color	ANT1	TALEN,CRISPR/Cas9	HR	Cermak et al.,2015
花序Inflorescence	L1L4	ZFN	NHEJ	Hilioti et al.,2016
	RRA3a	CRISPR/Cas9	NHEJ	Xu et al.,2015
	BOP	CRISPR/Cas9	NHEJ	Xu et al.,2016

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[1]	SHI Hongli, LI La, GUO Cuimei, YU Tingting, JIAN Wei, YANG Xingyong. Isolation，Identification and Analysis of Biocontrol Ability of Biocontrol Strain TL1 Against Tomato Botrytis cinerea [J]. Acta Horticulturae Sinica, 2023, 50(1): 79-90.
[2]	HU Jingyu, QUE Kaijuan, MIAO Tianli, WU Shaozheng, WANG Tiantian, ZHANG Lei, DONG Xian, JI Pengzhang, DONG Jiahong. Identification of Tomato Spotted Wilt Orthotospovirus Infecting Iris tectorum [J]. Acta Horticulturae Sinica, 2023, 50(1): 170-176.
[3]	ZHENG Jirong, WANG Tonglin, and HU Songshen. A New Tomato Cultivar‘Hangza 603’with High Quality [J]. Acta Horticulturae Sinica, 2022, 49(S2): 103-104.
[4]	ZHENG Jirong and WANG Tonglin. A New Tomato Cultivar‘Hangza 601’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 105-106.
[5]	ZHENG Jirong and WANG Tonglin. A New Cherry Tomato Cultivar‘Hangza 503’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 107-108.
[6]	HUANG Tingting, LIU Shuqin, ZHANG Yongzhi, LI Ping, ZHANG Zhihuan, and SONG Libo. A New Cherry Tomato Cultivar‘Yingshahong 4’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 109-110.
[7]	ZHANG Qianrong, LI Dazhong, QIU Boyin, LIN Hui, MA Huifei, YE Xinru, LIU Jianting, ZHU Haisheng, and WEN Qingfang. A New Tomato Cultivar‘Minnongke 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 73-74.
[8]	HAN Shuai, WU Jie, ZHANG Heqing, XI Yadong. Identification and Sequence Analysis of Tomato Spotted Wilt Orthotospovirus Infecting Lettuce in Sichuan [J]. Acta Horticulturae Sinica, 2022, 49(9): 2007-2016.
[9]	WU Fan, JIANG Junhao, LU Shan, ZHANG Nan, QIU Shuai, WEI Jianfen, SHEN Baichun. Research Progress on Germplasm Resources and Utilization of Hydrangea in China [J]. Acta Horticulturae Sinica, 2022, 49(9): 2037-2050.
[10]	CHEN Lilang, YANG Tianzhang, CAI Ruping, LIN Xiaoman, DENG Nankang, CHE Haiyan, LIN Yating, KONG Xiangyi. Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan [J]. Acta Horticulturae Sinica, 2022, 49(8): 1785-1794.
[11]	LIU Zhaoxia, ZHANG Xin, WANG Lu, MA Yuting, CHEN Qian, ZHU Zhanling, GE Shunfeng, JIANG Yuanmao. Effects of Fertilizer Hole Application Sites on Fine Root Growth,¹⁵N Absorption and Utilization,Yield and Quality of Apple Trees [J]. Acta Horticulturae Sinica, 2022, 49(7): 1545-1556.
[12]	LU Tao, YU Hongjun, LI Qiang, JIANG Weijie. Effects of Leaf and Fruit Quantity Regulation on Growth,Fruit Quality and Yield of Tomato [J]. Acta Horticulturae Sinica, 2022, 49(6): 1261-1274.
[13]	MENG Xianmin, CUI Qingqing, DUAN Yundan, ZHUANG Tuanjie, PU Dan, DONG Chunjuan, YANG Wencai, SHANG Qingmao. Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms [J]. Acta Horticulturae Sinica, 2022, 49(6): 1275-1289.
[14]	CUI Dongyu, LI Changqing, SUN Yanxin, WANG Jiqing, ZOU Guoyuan, YANG Jungang. Effects of Dwarf Close Planting on Growth and Yield of Tomato Under East-West Cultivation in Greenhouse [J]. Acta Horticulturae Sinica, 2022, 49(4): 875-884.
[15]	CHEN Tongqiang, ZHANG Tianzhu, WANG Xiaozhuo. Research Progress of The Regulation of Light on Lycopene Biosynthesis in Tomato Fruit [J]. Acta Horticulturae Sinica, 2022, 49(4): 907-923.