A Method for the Detection of cherry virus A（CVA）Based on Recombinase Polymerase Amplification（RPA）Technique

doi:10.16420/j.issn.0513-353x.2019-0566

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (2): 390-398.doi: 10.16420/j.issn.0513-353x.2019-0566

• New Technologies and New Methods • Previous Articles Next Articles

A Method for the Detection of cherry virus A（CVA）Based on Recombinase Polymerase Amplification（RPA）Technique

CHEN Ling，DUAN Xuwei，ZHANG Kaichun*，ZHANG Xiaoming，WANG Jing，YAN Guohua，and ZHOU Yu*

Key Laboratory of Urban Agricultural（North China），Ministry of Agriculture and Rural Affairs；Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China），Ministry of Agriculture and Rural Affairs；Beijing Academy of Forestry and Pomology Sciences，Beijing 100093，China

Online:2020-02-25 Published:2020-02-25

Abstract

Abstract: In this study，recombinase polymerase amplification（RPA）method was developed using the specific primers and probe designed based on the conserved gene sequences of cherry virus A（CVA）coat protein to detect CVA in cherry. The RPA method could be conducted under thermostotic condition at 38 ℃ and was completed within 40 min. The sensitivity test showed that the RPA was about 10-fold more sensitive than PCR and no cross-reactivity was detected with other 6 cherry-infected viruses and 1 viroid in China. Moreover，the terminally labeled amplicons could be visually inspected with lateral flow strip. This method was verified effectively by testing field-collected samples.

Key words: cherry, cherry virus A（CVA）, recombinase polymerase amplification（RPA）, detection

CLC Number:

S 662.5

CHEN Ling，DUAN Xuwei，ZHANG Kaichun*，ZHANG Xiaoming，WANG Jing，YAN Guohua，and ZHOU Yu*. A Method for the Detection of cherry virus A（CVA）Based on Recombinase Polymerase Amplification（RPA）Technique[J]. ACTA HORTICULTURAE SINICA, 2020, 47(2): 390-398.

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A Method for the Detection of cherry virus A（CVA）Based on Recombinase Polymerase Amplification（RPA）Technique

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