Abstract: In this study，total of 12 NAT（Nucleobase-Ascorbate Transporter）gene family members，named CaNAT01 to CaNAT12，were identified from pepper‘Zunla-1’（Capsicum annuum）by bioinformatics analysis. CaNATs were unevenly distributed on seven chromosomes and occurred segmental duplication tandem duplication events. Multiple transmembrane domains were identified in CaNATs. The isoelectric point of CaNATs were between 8.39 and 10.15. During the evolutionary process in plants，we found NATs originated from group D genes，then NATs stably existed in four groups of dicots after whole-genome duplication events and gene differentiation. The smallest nucleotide difference was found among group C genes，indicating that the genes in group C were more conservative. CaNATs showed tissue differential expression，some of which play vital roles in regulating root，stem，and fruit development in pepper. The transcript levels of CaNATs were generally up-regulated under multiple stresses（cold，heat，drought and salt），especially in cold and heat stresses.

Key words: pepper, NAT gene family, phylogenetic evolution, expression analysis