Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants

doi:10.16420/j.issn.0513-353x.2017-0188

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (12): 2399-2407.doi: 10.16420/j.issn.0513-353x.2017-0188

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Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants

WANG Weiying，LI Haiming，DAI Yimin，and LIN Jiangbo*

（Subtropical Agriculture Research Institute，Fujian Academy of Agricultural Sciences，Zhangzhou，Fujian 363005，China）

Online:2017-12-25 Published:2017-12-25

Abstract

Abstract:

To characterize the function of NtPLATZ1 gene from Narcissus tazetta var. chinensis，sense and antisense plant expression vectors were generated and transferred into Agrobacterium tumefaciens strain EHA105. Then vectors were transformed into tobacco plants by using Agrobacterium-mediated method. Regenerated plants of tobacco were detected by PCR-Southern blot and RT-PCR. The results showed that the sense and antisense genes were successfully integrated into the genomes of tobacco cells and expressed in transgenic tobacco plantlets. Transgenic plants were obtained successfully，which were inhibited the growth when the gene was over expression，and were promoted the growth when the gene was inhibited expression.

Key words: Narcissus tazetta var. chinensis, NtPLATZ1, expression vector, genetic transformation, tobacco

CLC Number:

S 682.2+1

WANG Weiying，LI Haiming，DAI Yimin，and LIN Jiangbo*. Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants[J]. ACTA HORTICULTURAE SINICA, 2017, 44(12): 2399-2407.

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Research on Construction of Sense and Antisense Plant Expression Vectors of NtPLATZ1 Gene in Narcissus tazetta var. chinensis and Genetic Transformation to Tobacco Plants

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