Genetic Diversity Analysis of Cordyceps militaris Using SSR Molecular#br# Markers

doi:10.16420/j.issn.0513-353x.2017-0290

ACTA HORTICULTURAE SINICA ›› 2017, Vol. 44 ›› Issue (11): 2195-2202.doi: 10.16420/j.issn.0513-353x.2017-0290

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Genetic Diversity Analysis of Cordyceps militaris Using SSR Molecular#br# Markers

HE Huaqi1，LIU Minxiang1，WANG Ying2，MAO Wenjun2，and BAO Dapeng2,*

1College of Life Science，Anhui Science and Technology University，Fengyang，Anhui 233100，China；2National
Engineering Research Center of Edible Fungi，Key Laboratory of Edible Fungi Resources and Utilization（South），Ministry
of Agriculture，Key Laboratory of Agricultural Genetics and Breeding of Shanghai，Institute of Edible Fungi，Shanghai
Academy of Agriculture Science，Shanghai 201403，China

Online:2017-11-25 Published:2017-11-25

Abstract

Abstract:

The Cordyceps militaris genome was used as template to identify the SSR loci，to design
the SSR primers，and the subsequent repeatable polymorphic bands were utilized for cluster analysis. The
results showed that 30 SSR primers generated 215 highly repeatable polymorphic bands. Based on the
estimation of genetic similarity coefficient（GS）calculated with unweighted pair group method arithmetic
average（UPGMA）clustering method，the variation range of GS values were from 0.527 to 0.995，and 46
C. militaris strains could be divided into 6 groups with a cutoff of the GS value of 0.761. There was a
certain correlation between the genetic relationship and the geographical origin of the strains.
Interestingly，the strains used for commercial cultivation were genetically similar to each other.

Key words: Cordyceps militaris, simple sequence repeats（SSR）, genetic similarity（GS）, cluster analysis

HE Huaqi1，LIU Minxiang1，WANG Ying2，MAO Wenjun2，and BAO Dapeng2,*. Genetic Diversity Analysis of Cordyceps militaris Using SSR Molecular#br# Markers[J]. ACTA HORTICULTURAE SINICA, 2017, 44(11): 2195-2202.

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Genetic Diversity Analysis of Cordyceps militaris Using SSR Molecular#br# Markers

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