Abstract: A intraspecific linkage map of Capsicum annuum was constructed from a recombinant inbred line population of F6 generation with 128 plants，which derived from a small fruit-sized hot pepper line‘Perennial’and a large fruit-sized sweet pepper line‘83-58’. In this map，18 linkage groups were generated with 131 markers（105 SSR markers，23 CAPS markers，1 SCAR marker and 2 morphological markers）. This genetic map covered 633.71 cM with an average intermarker distance of 4.8 cM. The length of linkage groups ranged from 7.07 to 75.16 cM with 3 to 17 markers per linkage group，and the average length reached 35.21 cM. Ten main agronomic taits of this population were collected for QTL mapping with QTL IciMapping V3.2 software，which was based on inclusive composite interval mappingmethod，and an additive effect model ICIM-ADD was selected in the QTL mapping. A total of 20 QTLs of 10 traits（stem length，plant elongation，first flower node，leaf length，leaf width，lateral bud，fruit shape，fruit shape index，fruit width，number of node）were mapped on the genetic map.

Key words: pepper, genetic linkage map, agronomic traits, QTL, RIL