Abstract: Six generations（P1，P2，F1，BC1，BC2 and F2）derived from the crosses of broccoli inbred line‘93219’（high susceptible）and the wild cabbage accession inbred line（Brassica macrocarpa Guss.）‘B2013’（high resistant）were used to investigate the inheritance of clubroot resistance by using mixed major gene plus polygene inheritance model. The results showed that the clubroot resistance was controlled by two additive-dominant-epitasis major genes（B-1 model）in the joint analysis of the six generations. The major genes heritability of BC1，BC2 and F2 were estimated to be 81.22%，78.36% and 80.00%，the genetic variance and the environmental variance accounted for 79.86% and 20.14% of the phenotypic variance in each population respectively. It indicated that the clubroot resistance was dominated by major genes though the environmental factors had a great effect. In practical breeding，higher heritability of major genes can be favorable to an efficient selection in early generations，such as BC1 and F2 for resistance to clubroot in broccoli breeding plan.

Key words: broccoli, wild cabbage accession, clubroot resistance, major gene, genetic analysis