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ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (10): 2094-2106.

• Other Horticulture Plants • Previous Articles     Next Articles

Correlation Between Spatiotemporal Profiles of Volatile Terpenoids and Relevant Terpenoid Synthase Gene Expression in Camellia sinensis

LIU Jing-jing1,*,WANG Fu-min1,*,LIU Guo-feng1,HE Zhi-rong2,YANG Hua1,WEI Chao-ling1,WAN Xiao-chun1,and WEI Shu1,**   

  1. 1Key Laboratory of Tea Biochemistry and Biotechnology,Anhui Agricultural University,Hefei 230036,China;2Food and Drug Administration of Youxian,Zhuzhou,Hunan 412300,China
  • Received:2014-05-05 Online:2014-10-25 Published:2014-10-25

Abstract: Monoterpenes and sesquiterpenes are the major tea aromatic compounds and important for tea quality. In this study,efforts were made to elucidate the molecular mechanism controlling volatile terpenoid biosynthesis in fresh tea leaves. Volatile terpenoid profiles in tea leaves and flowers were constructed using GC–MS technology. Tea leaves and flowers at different developmental stages exhibited distinct profiles of volatile terpenoids. Young leaves had more abundant terpenoids than old leaves,and theamount of glycosidically bound terpenoids were higher than free ones. Bioinformatics approaches were employed to map tea terpenoid synthase genes from previously obtained tea transcriptome data. Genes encoding for the following ten terpenoid synthases in tea were found:Linalool synthase,myrcene synthase,(E)-β-ocimene synthase,(R)-limonene synthase,(-)-α-terpineol synthase,(+)-α-phellandrene synthase,germacrene D synthase,(E,E)-α-farnesene synthase,linalool/nerolidol synthase and nerolidol/geranyl linalool synthase. Gene expression profiling revealed spatiotemporal patterns of the tested genes in tea plants. Moreover,their expression patterns were positively correlated to the abundance of volatile terpenoids. Additionally,the expression of six terpenoid synthase genes were induced by methyl jasmonate(MeJA),suggesting that emission profile of aromatic terpenoids might be enhanced in practice through these gene expression manipulation.

Key words: Camellia sinensis, fresh leaf, flower, terpenoid synthase gene, volatlie terpenoid biosynthesis, metabolic profiling, expression profile

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