Cloning of Chlorophyll a/b Binding Protein CmLhcb1 and Promoter from Chrysanthemum morifolium and Expression Analysis

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (6): 1119-.

• Ornamental Plants • Previous Articles Next Articles

Cloning of Chlorophyll a/b Binding Protein CmLhcb1 and Promoter from Chrysanthemum morifolium and Expression Analysis

HAN Shuang1,2，LIU Rui-xia1，ZHANG Zhao-he1，CHEN Su-mei1，JIANG Jia-fu1，FANG Wei-min1，LIAO Yuan1，and CHEN Fa-di1,*

（1Department of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2College of Life Science，Shangqiu Normal University，Shangqiu，Henan 476000，China）

Received:2013-02-26 Revised:2013-05-15 Online:2013-06-25 Published:2013-06-25

Abstract

Abstract: The cDNA of cut chrysanthemum‘Gongzi’was used to clonehomologous gene cab，which had 798 bp ORF and 266 amino acid. After blast analysis，we confirmed that the gene was ranked as Lhcb1，and was named as CmLhcb1. Using the cDNA of‘Puma Sunny’chrysanthemum to homologously clone gene Lhcb1，we got the same amino sequence with that of‘Gongzi’. The expression of CmLhcb1 was the higher in leaf than that in stem，flower and root. Low light and GA₃ treatment increased CmLhcb1 expression. Paclobutrazol treatment inhibited CmLhcb1 expression.The circadian clock regulated the expression of CmLhcb1. The gene expression in day was enormously higher than that in night. The promoter sequence 715 bp of‘Gongzi’cut chrysanthemum and 716 bp of‘Puma Sunny’were cloned using high-efficiency TAIL-PCR（hiTAIL-PCR），and many biologic and abiotic stress responsive elements related to light，GA，ABA，water，SA and virus were found by PLACE Databank. The promoter was light responsive，and it had the GT1-box and Z-box element.

Key words: Chrysanthemum morifolium, chlorophyll a/b-binding protein（LHC）, promoter, clone

CLC Number:

S 682.1+1

HAN Shuang1,2，LIU Rui-xia1，ZHANG Zhao-he1，CHEN Su-mei1，JIANG Jia-fu1，FANG Wei-min1，LIAO Yuan1，and CHEN Fa-di1,*. Cloning of Chlorophyll a/b Binding Protein CmLhcb1 and Promoter from Chrysanthemum morifolium and Expression Analysis[J]. ACTA HORTICULTURAE SINICA, 2013, 40(6): 1119-.

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Cloning of Chlorophyll a/b Binding Protein CmLhcb1 and Promoter from Chrysanthemum morifolium and Expression Analysis

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