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ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (3): 498-504.

• Vegetables • Previous Articles     Next Articles

Transformation of Broccoli with KTI Gene and the Bioassay for
Diamondback Moth Resistance

JIANG  Han-Min, SONG  Wen-Qin, LIU  Li-Li, WEN  Zheng-Hua, YAO  Xing-Wei, DAN  Xiao-Zheng, SUN  De-Ling   

  1. (1Tianjin Kernel Vegetable Research Institute,Tianjin 300384,China;2College of Life Sciences,Nankai University,
    Tianjin 300071,China)
  • Online:2013-03-25 Published:2013-03-25

Abstract: KTI gene was transformed into broccoli(Brassica oleracea L. ssp. italica)genome via
Agrobacterium mediated transformation method using broccoli lines‘09LR-11’as explants. Thirteen
kanamycin resistant broccoli plants were obtained. PCR analysis with primers specific to KTI gene
identified 8 KTI positive plants. Southern blotting analysis with KTI gene as probes showed that the KTI
gene was integrated into broccoli genome. RT-PCR analysis indicated that the KTI gene was expressed in
transgenic broccoli. Both laboratory and field tests proved that the transgenic broccoli plants were resistant
to diamondback moth(Plutella xylostella larvae).

Key words: broccoli, KTI gene, Plutella xylostella larvae, insect resistance, genetic transformation

CLC Number: