Abstract: The study took one wild germplasm of Rosa rugosa as female parent，two cultivars of R. rugosa and two cultivars of rose as male parents. After the hybridization，we took the hybridized styles to culture and empolyed fluorescent microscope to observe pollen tube growth in the styles. The results indicated that 15% sucrose，0.005% H₃BO₃，0.1% agar，and pH 7.0，were optimal for pollen tube growth. If we excised hybridized styles to culture when it reached 4 hours after pollination，the consequent was better. For the interspecific hybridized combinations，the effect of style cutting length on the results of semi vitro culture of style was significant. There was only a small amount of pollen tubes through the incisions when we cultured one third-length styles. To guarantee good pollination result，the germination rate of pollen which was used for pollination must be above 40%.

Key words: Rosa rugosa, rose, semi vitro culture of style, fluorescent microscope observation