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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (6): 1107-1114.

• Vegetables • Previous Articles     Next Articles

Identification of Tomato spotted wilt virus and Screening for Resistant Sources in Tomato

QIU  Shu-Liang, WANG  Xiao-Xuan, DU  Yong-Chen, GAO  Jian-Chang, GUO  Yan-Mei, ZHU  De-Wei, HU  Hong, LI  Bao-Ju, SHI  Yan-Xia   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2012-06-25 Published:2012-06-25

Abstract: Fifteen tomato plants which seemed infected Tomato spotted wilt virus(TSWV)were selected randomly from the tomato cultivation greenhouse of Shunyi District,Beijing. Then the leaves and fruits were obtained from each plant and tested for the presence of TSWV using a DAS-ELISA kit. The results showed positive,indicating that 15 tomato materials had been infected with TSWV. A genetic analysis of 442 tomato materials with co-dominant SCAR markers developed from Sw-5,TSWV resistance gene originated from Solanum peruvianum,was issued and identified 24 resistant materials,which including 4 wild tomatoes,1 hybrid,16 breeding inbred lines and 3 S. pennellii introgression lines. Those 4 wild tomatoes are not suitable as breeding materials because of the poor agronomic characters,i.e. small fruits, low production,poor quality and bad flavor,while the other 20 tomato materials can be used for developing cultivars resistant to TSWV due to their better agronomic characters.

Key words: tomato, Tomato spotted wilt virus, Sw-5, resistance breeding

CLC Number: