Genetic Analysis of Triploid Progenies from Shatian Pummelo（2x）× Citrus Allotetraploid Somatic Hybrid NS（4x）

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (6): 1021-1026.

• Fruit Trees • Next Articles

Genetic Analysis of Triploid Progenies from Shatian Pummelo（2x）× Citrus Allotetraploid Somatic Hybrid NS（4x）

SONG Jian-Kun, ZHU Shi-Ping, TAN Mei-Lian, GUO Wen-Wu, DENG Xiu-Xin

Key Laboratory of Horticultural Plant Biology（Ministry of Education），College of Horticulture & Forestry，Huazhong Agricultural University，Wuhan 430070，China

Online:2012-06-25 Published:2012-06-25

Abstract

Abstract: Two pairs of SSR（simple sequence repeat）primers TAA1 and TAA3 were used to detect the band patterns and segregation rations among the 79 triploid progenies derived from the sexual cross between diploid Citrus grandis（L.）Osbeck‘Shatian’pummelo and somatic hybrid NS [（C. reticulata
Blanco × C. paradisi Macf.）‘Nova’tangelo + C. sinensis（L.）Osbeck‘Succari’sweet orange] by embyo rescue technology. Five and four band patterns were generated from the TAA1 and TAA3 primers at the ratio of 4︰1︰1︰5︰1 and 2︰2︰1︰1 respectively，as was in good accordance with the segregation ratio of amphidiploid deduced by the law of Mendel. The results preliminarily indicated that the meiosis behavior of citrus allotetraploid somatic hybrid was similar to that of the amphidiploid.

Key words: citrus, allotetraploid somatic hybrid, SSR marker, genetic analysis, triploid progeny, amphidiploid

CLC Number:

S 666

SONG Jian-Kun, ZHU Shi-Ping, TAN Mei-Lian, GUO Wen-Wu, DENG Xiu-Xin. Genetic Analysis of Triploid Progenies from Shatian Pummelo（2x）× Citrus Allotetraploid Somatic Hybrid NS（4x）[J]. ACTA HORTICULTURAE SINICA, 2012, 39(6): 1021-1026.

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Genetic Analysis of Triploid Progenies from Shatian Pummelo（2x）× Citrus Allotetraploid Somatic Hybrid NS（4x）

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