Abstract: A population BC₂S₆ with 152 lines was developed from the cross between Solanum lycopersicum 9706 and Solanum cheesmanii LA0317. A linkage map was constructed with 155 SSR marker，21 CAPS marker and 88 Indel marker. The map covered 1 134.0 cM，and the average distance between adjacent loci was 4.29 cM．Using MQM-mapping method，QTL of soluble solids content（SSC）and Beta-carotene content were detected. Ten QTL of SSC were mapped to chromosome 3，4，5，6，9，10 and 11. Respectively，five of which explained more than 12% of the phenotypic variation. Six QTL of Beta-carotene content were mapped to chromosome 1，2，6，9. The major QTL of Beta-carotene content on chromosome 6 could explain 54.7% of the phenotypic variation．Moreover，5 lines with high SSC and 8 lines with high Beta-carotene content were obtained，and could be used as breeding materials.

Key words: tomato, Solanum cheesmanii, soluble solids content, Beta-carotene content, QTL