Full Length cDNA Cloning and Expression Analysis of UFGT Gene from Ginkgo biloba

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (10): 1903-1912.

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Full Length cDNA Cloning and Expression Analysis of UFGT Gene from Ginkgo biloba

ZHANG Chuan-Li, CHEN Peng, ZHONG Yue-Ming, ZHOU Chang-Yuan, SHEN Dan-Hong, JIANG Fei

（1School of Horticulture and Plant Protection，Yangzhou University，Yangzhou，Jiangsu 225009，China；2College of Bioscience and Biotechnology，Yangzhou University，Yangzhou，Jiangsu 225009，China）

Online:2012-10-25 Published:2012-10-25

Abstract

Abstract: A full length cDNA of UDP-glycose：flavonoid glycosyltransferase gene was obtained from ginkgo leaf for the first time，using degenerate primer PCR and RACE protocol. The sequence which was designated as GbUFGT，had been embodied in GenBank database with an accession No. of JN640564.2. The cDNA sequence and genomic DNA sequence of GbUFGT were the same，in other words，GbUFGT was an intron-less gene. The coding region of GbUFGT was 1 491 bp long，encoding 496 amino acids. The deduced GbUFGT protein containing a typical conversed plant secondary product glycosyltransferase（PSPG）motif，and an UDP-glucoronosyltransferase and UDP-glucosyltransferase domain，showed high identities to other plant UFGTs. Semi-quantitative RT-PCR analysis revealed that GbUFGT expressed highly at the whole developmental stages of ginkgo leaf，and the expression levels were similar in different developmental periods，displaying developmental-stage independence.

Key words: Ginkgo biloba, UDP-glycose：flavonoid glycosyltransferase（UFGT）, cDNA, genomic DNA, expression pattern

CLC Number:

S 664.3

ZHANG Chuan-Li, CHEN Peng, ZHONG Yue-Ming, ZHOU Chang-Yuan, SHEN Dan-Hong, JIANG Fei. Full Length cDNA Cloning and Expression Analysis of UFGT Gene from Ginkgo biloba[J]. ACTA HORTICULTURAE SINICA, 2012, 39(10): 1903-1912.

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Full Length cDNA Cloning and Expression Analysis of UFGT Gene from Ginkgo biloba

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