Determination of Sulforaphane by High Performance Liquid Chromato- graphy and Genetic Analysis of DH Population in Broccoli Florets

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (1): 101-108.

• Vegetables • Previous Articles Next Articles

Determination of Sulforaphane by High Performance Liquid Chromato- graphy and Genetic Analysis of DH Population in Broccoli Florets

LI Zhan-sheng1，LIU Yu-mei1,*，FANG Zhi-yuan1，YANG Li-mei1，ZHUANG Mu1，ZHANG Yang-yong1，YUAN Su-xia1，ZHAO Wen2，LIU Er-yan1，and SUN Pei-tian1

（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Supervision and Testing Center for Vegetable Quality，Ministry of Agriculture，Beijing 100081，China）

Received:2011-09-02 Revised:2011-11-24 Online:2012-01-25 Published:2012-01-25
Contact: LIU Yu-mei

Abstract

Abstract: A doubled haploid（DH）population derived from broccoli combination of inbred lines 86101 and 90196 was used to investigate the inheritance of sulforaphane content in broccoli（Brassica oleracea L. var. italica Planch.）florets by high performance liquid chromatography（HPLC）and mixed major gene plus polygene inheritance model. The population consists of 176 DH lines was obtained by F1 microspore culture，and the trial was set by random complete block design with three replications. The optimal model was three major genes plus polygenes. The heritability of major genes and polygenes was 89.28% and 2.58% individually which showed major genes had a key role in controlling sulforaphane content. At the same time，major genes followed addictive effect and epistatic effect.

Key words: broccoli, DH population, sulforaphane, genetic analysis, high performance liquid chromatography（HPLC）

CLC Number:

S 635

LI Zhan-sheng;LIU Yu-mei;FANG Zhi-yuan;YANG Li-mei;ZHUANG Mu;ZHANG Yang-yong;YUAN Su-xia;ZHAO Wen;LIU Er-yan;and SUN Pei-tian. Determination of Sulforaphane by High Performance Liquid Chromato- graphy and Genetic Analysis of DH Population in Broccoli Florets[J]. ACTA HORTICULTURAE SINICA, 2012, 39(1): 101-108.

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Determination of Sulforaphane by High Performance Liquid Chromato- graphy and Genetic Analysis of DH Population in Broccoli Florets

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