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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (7): 1308-1316.

• Vegetables • Previous Articles     Next Articles

A Microsatellite-based Genetic Map of Melon and Localization of Gene for Gynoecious Sex Expression Using Recombinant Inbred Lines

GAO Mei-ling1,2,ZHU Zi-cheng1,GAO Peng1,and LUAN Fei-shi1,*   

  1. (1 College of Horticulture,Northeast Agricultural University,Harbin 150030,China;2College of Life Science,Agriculture and Forestry,Qiqihaer University,Qiqihaer,Heilongjiang 161006,China)
  • Received:2011-03-30 Revised:2011-05-31 Online:2011-07-25 Published:2011-07-25
  • Contact: LUAN Fei-shi1,*

Abstract: A recombinant inbred line(RIL)mapping population of melon,Cucumis melo L. comprising of 185 F6︰7 families was developed from a cross between the monoecious inbred line 3-2-2(AAGG)and the gynoecious inbred line WI998(AAgg)through single-seed descent(SSD). This RIL population was used to develop a linkage map with microsatellite(or simple sequence repeat,SSR)markers. Of 1 219 SSR markers screened,215(17.6%)were polymorphic between the two parental lines which were applied for linkage analysis in 185 RILs. The resulting genetic map consisted of 18 linkage groups spanning 937.1 cM with a mean marker interval of 4.4 cM. Two SSR markers,NR3 and MU8294_1 were identified flanking the g locus in linkage group 1 which was 1.2 cM and 2.6 cM away from the gynoecy gene,respectively.

Key words: melon, Cucumis melo L., RIL, SSR, genetic map, gynoecious, localization of gene

CLC Number: