Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (6): 1153-1160.

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Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences

XU Lan-zhen，LIU Xiao-feng，HE Yong-rui，YAO Li-xiao，PENG Ai-hong，LEI Tian-gang，and CHEN Shan-chun*

（Citrus Research Institute，Chinese Academy of Agricultural Sciences/Southwest University，National Citrus Improvement Center，National Citrus Engineering Research Center，Chongqing 400712，China）

Received:2010-09-25 Revised:2011-05-20 Online:2011-06-25 Published:2011-06-25
Contact: CHEN Shan-chun

Abstract

Abstract: In order to analyze the differentially expressed genes in the post-harvested citrus fruit，a subtracted cDNA library was constructed by suppression subtractive hybridization（SSH）between the fruit pulp of post-storage（experimental group，the Tester）and that of pre-storage（control group，the Driver）. The library included about 656 clones which were 100–1 000 bp in size，among which 213 clones were selected randomly and sequenced. Totally 143 useful ESTs were acquired，and 53 uniESTs were finally obtained. Sequence similarity searches via BLASTx and BLASTn on NCBI showed that the ESTs were sorted into 14 functional categories according to the Munich Information Center for Protein Sequences（MIPS）. The ESTs involved in metabolism，energy，protein synthesis accounted for 17%，11% and 13% of the total ESTs，respectively.

Key words: citrus, fruit, granulation, suppression subtractive hybridization, EST

CLC Number:

S 666

XU Lan-zhen;LIU Xiao-feng;HE Yong-rui;YAO Li-xiao;PENG Ai-hong;LEI Tian-gang;and CHEN Shan-chun . Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences [J]. ACTA HORTICULTURAE SINICA, 2011, 38(6): 1153-1160.

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Isolation of Granulation-related ESTs in Newhall Navel Orange Fruit Storage and Primary Analyses of the Sequences

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