Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis（L.）Osbeck]

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (5): 859-866.

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Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis（L.）Osbeck]

CHENG Chun-zhen 1,2，ZHU Shi-ping 1,2，WU Bo 1,2，YANG Jia-wei 1,3，BEI Xue-jun 1,2，MA Yan-yan 1,2，and ZHONG Guang-yan 1,2,*

（¹ College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716，China；²Citrus Research Institute，Chinese Academy of Agricultural Sciences/ Southwest University，Chongqing 400712，China；³ Kaixian Fruit Technology Extension Station，Chongqing 405400，China）

Received:2011-01-21 Revised:2011-05-06 Online:2011-05-25 Published:2011-05-25
Contact: ZHONG Guang-yan

Abstract

Abstract: A suppression subtractive hybridization library was successfully constructed using cDNA synthesized from RNA extracted from peels of‘Liangping’pemmelo[Citrus grandis（L.）Osbeck] receiving UV-B radiation as tester and non-UVB treatment as driver. Two hundred randomly-picked positive clones were sequenced，and a total of 168 EST sequences were obtained. One hundred and fourteen ESTs，involving in 57 genes，were found to share considerable homology with known genes while the rest 54 ESTs had low or even no homology with known genes. Function analysis showed that these differentially expressed genes were related to numerical celluar events such as stress-tolerance，growth and development，cell apoptosis，cell differenciation，gene transcription and translation，signaling，energy metabolism，carbohydrate and amino acid metabolism and secondary metabolism.

Key words: citrus, ultravolet radiation, suppression subtractive hybridization, expressed sequence tags, gene ontology

CLC Number:

S 666

CHENG Chun-zhen;ZHU Shi-ping;WU Bo;YANG Jia-wei;BEI Xue-jun;MA Yan-yan;and ZHONG Guang-yan;. Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis（L.）Osbeck] [J]. ACTA HORTICULTURAE SINICA, 2011, 38(5): 859-866.

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Effect of UV-B Radiation on Gene Expression in Peels of‘Liangping’Pommelo[Citrus grandis（L.）Osbeck]

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