Abstract: To analyze the relationship of sugar accumulation with sugar-related gene expression during strawberry fruit development，receptacle of‘Fugilia’strawberry（Fragaria × ananassa Duch.）was used and real-time RT-PCR primers were designed to analyze both changes of the mRNA expression of four sugar accumulation-related genes and soluble sugar contents，respectively. The main results showed that sucrose，glucose and fructose contents increased continually during fruit development，in particular sucrose accumulation was closely related to fruit ripening. The mRNA expression level of sucrose phosphate synthase（SPS）gene increased gradually during fruit development，and a rapid increase occurred in later developmental stages；The mRNA expression level of sucrose synthase（SS）and acid invertase（AI）decreased continually in addition to white or turning stages；The mRNA expression level of sucrose transporter（SUT1）displayed increased firstly，and then declined，finally increased again，in particular increased rapidly before white period. Combinational analysis of the changes of soluble sugars content and gene expression demonstrated that sucrose carrier and sucrose phosphate synthase play an important role in regulation of strawberry fruit ripening.

Key words: strawberry, fruit, development, soluble sugars, sucrose transporter, real-time RT-PCR