Utility of Arabidopsis Chloroplast Simple Sequence Repeat（cpSSR）Primers in Cabbage（Brassica oleracea L. var. capitata L.）

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (3): 549-555.

• Research Notes • Previous Articles Next Articles

Utility of Arabidopsis Chloroplast Simple Sequence Repeat（cpSSR）Primers in Cabbage（Brassica oleracea L. var. capitata L.）

ZHANG Yang-yong*，FANG Zhi-yuan，WANG Qing-biao，LIU Yu-mei，YANG Li-mei，ZHUANG Mu，and SUN Pei-tian

Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2010-09-13 Revised:2011-01-19 Online:2011-03-25 Published:2011-03-25
Contact: ZHANG Yang-yong

Abstract

Abstract: According to the Arabidopsis thaliana chloroplast whole genome sequence，89 chloroplast SSRs（cpSSRs）were searched，with an average of 1.736 kb per SSR. Thirty-two pairs of primers，out of 58 designed primers got effective amplification in cabbage. Between cabbage C300，C322 and rapeseed polima N478，N479，five primers showed polymorphism，which located in intron or intergenic region. The number of single nucleotide repeat A/T ranged from 8 to 13. Homology between C300 and N479 was greater than the homology between Arabidopsis thaliana and C300，N479.

Key words: Cabbage, cpSSR, polima rapeseed, polymorphism

CLC Number:

S 635

ZHANG Yang-yong;FANG Zhi-yuan;WANG Qing-biao;LIU Yu-mei;YANG Li-mei;ZHUANG Mu;and SUN Pei-tian . Utility of Arabidopsis Chloroplast Simple Sequence Repeat（cpSSR）Primers in Cabbage（Brassica oleracea L. var. capitata L.） [J]. ACTA HORTICULTURAE SINICA, 2011, 38(3): 549-555.

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Utility of Arabidopsis Chloroplast Simple Sequence Repeat（cpSSR）Primers in Cabbage（Brassica oleracea L. var. capitata L.）

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