Construction of the Suppression Subtractive Hybridization Library and Analysis of Related Genes of Floral Buds in Prunus persica During Dormancy-releasing

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (12): 2273-2280.

• Fruit Trees • Previous Articles Next Articles

Construction of the Suppression Subtractive Hybridization Library and Analysis of Related Genes of Floral Buds in Prunus persica During Dormancy-releasing　　

LI Ling，WANG Hui，TAN Yue，WANG Yu，CHEN Xiu-de，LI Dong-mei，and GAO Dong-sheng*

（College of Horticulture Science and Engineering，State Key Laboratory of Crop Biology，Shandong Agricultural University，Tai’an，Shandong 271018，China）

Received:2011-07-17 Revised:2011-12-05 Online:2011-12-25 Published:2011-12-25
Contact: GAO Dong-sheng

Abstract

Abstract: To better understand the molecular and physiological mechanisms of seasonal bud dormancy in perennial trees，the differentially expressed genes in peach[Prunus persica（L.）Batsch]floral buds during dormancy-releasing were identified by suppression subtractive hybridization（SSH）. One hundred and eighty positive clones were selected and sequenced from the libraries totally，and 28 unique genes were obtained. According to the BLAST screening and functional annotation，metabolism，oxidation reduction，signal transduction and defense-related genes were identified with high frequencies in fertility cDNA library. In SSH library three representative genes，HisH3，Dhn and Metallothionein were selected and analyzed qRT-PCR. The results showed that they were up-regulated during dormancy-releasing of floral buds.

Key words: peach, floral bud, dormancy, suppression subtractive hybridization（SSH）, gene expression, qRT-PCR

CLC Number:

S 662.1

LI Ling;WANG Hui;TAN Yue;WANG Yu;CHEN Xiu-de;LI Dong-mei;and GAO Dong-sheng. Construction of the Suppression Subtractive Hybridization Library and Analysis of Related Genes of Floral Buds in Prunus persica During Dormancy-releasing　　[J]. ACTA HORTICULTURAE SINICA, 2011, 38(12): 2273-2280.

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Construction of the Suppression Subtractive Hybridization Library and Analysis of Related Genes of Floral Buds in Prunus persica During Dormancy-releasing

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