Screening and Analysis of BAC Clones Containing Flowering Time Gene FLC1 in Brassica campestris L. ssp. pekinensis Olsson

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (9): 1513-1516.

• 研究报告 • Previous Articles Next Articles

Screening and Analysis of BAC Clones Containing Flowering Time Gene FLC1 in Brassica campestris L. ssp. pekinensis Olsson

SHI Xue-ping，FENG Da-ling，WANG Yan-hua，XUAN Shu-xin，and SHEN Shu-xing*

（College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China）

Received:2010-04-23 Revised:2010-06-17 Online:2010-09-25 Published:2010-09-25
Contact: SHEN Shu-xing

Abstract

Abstract: The primary and secondary pools of a bacterial artificial chromosome（BAC）library in Chinese cabbage were constructed using improved BAC pooling strategy. The specific primers of flowering time gene FLC1 were to screen those BAC clones by PCR amplification. In total two positive FLC1 clones were obtained from 19 200 BACs through three steps of PCR screening with 114 reactions. Sequence analysis of FLC1 clones showed that these fragments has 99% of homology with the FLC1 in Chinese cabbage，indicating that the flowering time gene FLC1 is contained in the BAC clones.

Key words: Chinese cabbage, BAC clone, FLC1 gene, pool, PCR screening

CLC Number:

S 634.1

SHI Xue-ping;FENG Da-ling;WANG Yan-hua;XUAN Shu-xin;and SHEN Shu-xing. Screening and Analysis of BAC Clones Containing Flowering Time Gene FLC1 in Brassica campestris L. ssp. pekinensis Olsson[J]. ACTA HORTICULTURAE SINICA, 2010, 37(9): 1513-1516.

[1]	HAN Shuhui, HAN Caifeng, HAN Shurong, HAN Caimei, and HAN Xu. A New Autumn Chinese Cabbage Hybrid‘Jiaoyan Qiubao’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 83-84.
[2]	WANG Weihong, ZHANG Fenglan, YU Yangjun, ZHANG Deshuang, ZHAO Xiuyun, YU Shuancang, SU Tongbing, LI Peirong, and XIN Xiaoyun. A New Chinese Cabbage Cultivar‘Jingqiu 1518’for Autumn Cultivation [J]. Acta Horticulturae Sinica, 2022, 49(S2): 85-86.
[3]	YU Yangjun, WANG Weihong, SU Tongbing, ZHANG Fenglan, ZHANG Deshuang, ZHAO Xiuyun, YU Shuancang, LI Peirong, XIN Xiaoyun, and WANG Jiao. A New Chinese Cabbage Cultivar‘Jingchun CR3’with Clubroot Resistance and Bolting Tolerance [J]. Acta Horticulturae Sinica, 2022, 49(S2): 87-88.
[4]	WANG Lili, WANG Xin, WU Haidong, WEN Qiang, and Yang Xiaofei. A New Chinese Cabbage Cultivar‘Liaobai 28’with Resistance to Clubroot Disease [J]. Acta Horticulturae Sinica, 2022, 49(S2): 89-90.
[5]	YU Yangjun, SU Tongbing, ZHANG Fenglan, ZHANG Deshuang, ZHAO Xiuyun, YU Shuancang, WANG Weihong, LI Peirong, XIN Xiaoyun, WANG Jiao, and WU Changjian. A New Purple Seedling-Edible Chinese Cabbage F1 Hybrid‘Jingyan Zikuaicai’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 91-92.
[6]	HUANG Li, CHEN Caizhi, YU Xiaolin, YAO Xiangtan, and CAO Jiashu, . A New Early-mid Maturing Chinese Cabbage Pak-choi Cultivar‘Zhedaqing’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 93-94.
[7]	XU Ligong, HAN Taili, SUN Jifeng, YANG Xiaodong, and TAN Jinxia. A New Seedling-edible Chinese Cabbage Cultivar‘Jinlü 2’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 67-68.
[8]	WANG Yu, ZHANG Xue, ZHANG Xueying, ZHANG Siyu, WEN Tingting, WANG Yingjun, GAN Caixia, PANG Wenxing. The Effect of Camalexin on Chinese Cabbage Resistance to Clubroot [J]. Acta Horticulturae Sinica, 2022, 49(8): 1689-1698.
[9]	ZHANG Lugang, LU Qianqian, HE Qiong, XUE Yihua, MA Xiaomin, MA Shuai, NIE Shanshan, YANG Wenjing. Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage [J]. Acta Horticulturae Sinica, 2022, 49(7): 1582-1588.
[10]	QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool [J]. Acta Horticulturae Sinica, 2022, 49(3): 613-621.
[11]	WANG Guangpeng, LIU Tongkun, XU Xinfeng, LI Zhubo, GAO Zhanyuan, HOU Xilin. Identification of LEA Family and Expression Analysis of Some Members Under Low-temperature Stress in Chinese Cabbage [J]. Acta Horticulturae Sinica, 2022, 49(2): 304-318.
[12]	WANG Ronghua, WANG Shubin, LIU Shuantao, LI Qiaoyun, ZHANG Zhigang, WANG Lihua, ZHAO Zhizhong. Transcriptome Analysis of Waxy Near-isogenic Lines in Chinese Cabbage Floral Axis [J]. Acta Horticulturae Sinica, 2022, 49(1): 62-72.
[13]	XU Ligong, HAN Taili, SUN Jifeng, YANG Xiaodong, TAN Jinxia, SONG Yinhang, LI Meng, ZHOU Luhong, and SUN Shasha. A New Spring Chinese Cabbage Cultivar‘Weichun 22’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2825-2826.
[14]	WANG Weihong, ZHANG Fenglan, YU Yangjun, ZHANG Deshuang, ZHAO Xiuyun, YU Shuancang, SU Tongbing, LI Peirong, and XIN Xiaoyun. A New Chinese Cabbage Cultivar‘Jingqiu 5’for Autumn Cultivation [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2827-2828.
[15]	WANG Mingqiu, MENG Chuan, MU Jingui, WANG Yuhai, LIU Xuemin, CHEN Zhanliang, and LIU Xiaodong. A New Chinese Cabbage Cultivar‘Jibai 6’with Disease Resistance [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2829-2830.

Screening and Analysis of BAC Clones Containing Flowering Time Gene FLC1 in Brassica campestris L. ssp. pekinensis Olsson

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments