Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (4): 575-580.

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Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum

ZHANG Lu1，DING Yan-qin2，DU Bing-hai2，WEI Min1,*，and WANG Xiu-feng1

（1College of Horticultural Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology， Tai’an，Shandong 271018，China；2College of Life Sciences，Shandong Agricultural University，Tai’an，Shandong 271018，China）

Received:2009-11-24 Revised:2010-03-15 Online:2010-04-25 Published:2010-04-25
Contact: WEI Min

Abstract

Abstract: The taxonomic status, growth promoting and biocontrol effects against Fusarium oxysporum f. sp. cucumerinum of antagonistic bacterium DS-1 strain, screened by co-culture on PDA plates from rhizospheric soil of continuously cropped cucumber in solar greenhouse, morphology, physiological and biochemical characteristics, and 16S rDNA sequencing of the strain were determined, the effects on cucumber seed germination and seedling fusarium wilt biocontrol were studied. The results showed that: The 16S rDNA sequence of DS-1 strain shared 100% homology with Brevibacillus brevis from GenBank. Based on the results of 16S rDNA sequencing, morphological and cultural observation as well as physiological and biochemical determination, DS-1 strain was identified as Brevibacillus brevis, with GenBank accession number EF108303. DS-1 strain promoted germination of cucumber seeds, with significant biocontrol effects against fusarium wilt of cucumber seedlings by 84.92%.

Key words: cucumber, Fusarium oxysporum f. sp. cucumerinum, antagonistic bacterium, identification, Brevibacillus brevis

CLC Number:

S 642.2

ZHANG Lu;DING Yan-qin;DU Bing-hai;WEI Min;and WANG Xiu-feng. Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum[J]. ACTA HORTICULTURAE SINICA, 2010, 37(4): 575-580.

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Identification and Biocontrol Effects of Antagonistic Bacterium DS-1 Strain Against Fusarium oxysporum f. sp. cucumerinum

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