The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var. capitata L. and Male Sterile Line in B. juncea

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (10): 1661-1666.

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The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var. capitata L. and Male Sterile Line in B. juncea

WANG Tao-tao，CAI Xiao-feng，ZHANG Jun-hong，LI Han-xia，and YE Zhi-biao*

（Key Laboratory of Horticultural Plant Biology，Ministry of Education，Huazhong Agricultural University，College of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China）

Received:2010-06-13 Revised:2010-08-24 Online:2010-10-25 Published:2010-10-25
Contact: YE Zhi-biao

Abstract

Abstract: The development of the distant hybrid embryos derived from Brassica oleracea var. capitata L. and male sterile line of B. juncea were investigated in this study. The results showed that the hybrid embryos began to abort in approximate 10 days after pollination. Some hybrid plantlets were obtained by using ovule rescue culture in vitro in MS media supplemented with 1 mg · L-1 BA，0.1 mg · L-1 NAA and activated charcoal. The development of anther in distant hybrid plants were examined using paraffin section，and the abortion was found in the differentiation stage of stamen primordium.

Key words: Brassica juncea, male sterile, cabbage, ovule rescue culture

CLC Number:

S 635

WANG Tao-tao;CAI Xiao-feng;ZHANG Jun-hong;LI Han-xia;and YE Zhi-biao. The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var. capitata L. and Male Sterile Line in B. juncea[J]. ACTA HORTICULTURAE SINICA, 2010, 37(10): 1661-1666.

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The Culture and Early Male Sterile Identification of Distant Hybrid Embryos Derived from Brassica oleracea var. capitata L. and Male Sterile Line in B. juncea

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