Cloning and Analysis of the Promoter of SlIAA14 in Tomato

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (10): 1605-1612.

• Vegetables • Previous Articles Next Articles

Cloning and Analysis of the Promoter of SlIAA14 in Tomato

LI Xiao-jing1，CHEN Yin-hua1，ZHANG Jun-hong2,*，and YE Zhi-biao2

（1College of Agriculture，Hainan University，Haikou 570228，China；2College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China）

Received:2010-05-25 Revised:2010-08-28 Online:2010-10-25 Published:2010-10-25
Contact: ZHANG Jun-hong

Abstract

Abstract: Promoter analysis of SlIAA14 was carried out，to further study the expression profile of SlIAA14 and its regulatory mechanism in tomato root development. A 2 197 bp promoter fragment was cloned from tomato cultivar Ailsa Craig，using PCR-based genomic DNA walking. Several putative cis-elements responding to phytohormones（ABA，GA and CTK）and stress（dehydration and wounding）were predicted using online promoter analysis software. This promoter fragment was employed to drive YFP-GUS reporter genes，and was introduced into tomato using Agrobacterium-mediated transformation. YFP and GUS activity was detected in tomato root tip and lateral root primordium，suggesting SlIAA14 gene might participate in the root elongation and lateral root initiation of tomato.

Key words: tomato, SlIAA14, promoter, root, development

CLC Number:

S 641.2

LI Xiao-jing;CHEN Yin-hua;ZHANG Jun-hong;and YE Zhi-biao. Cloning and Analysis of the Promoter of SlIAA14 in Tomato[J]. ACTA HORTICULTURAE SINICA, 2010, 37(10): 1605-1612.

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Cloning and Analysis of the Promoter of SlIAA14 in Tomato

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