Abstract: The influence of genotype，hormone，AgNO3 and low temperature (4 ℃) on anther culture of carrot (Daucus carota L.) was investigated with 7 open pollinated cultivars，10 inbred lines，16 F1 and 6 F2. Microspore embryogenesis occurred on six accessions and the highest frequency was 50071 with 3.89%. Thirty accessions formed the calli derived from microspores and 600Q6 had the highest frequency with 36.70%. On the B5 media with 2,4-D and NAA，all of four tested accessions formed the calli，and both of 50071 and 70Q67 formed the microspores-derived embryos，but none on the media with 6-BA and NAA. 50071 and 70Q67 were induced about 10.00% and 2.00% of embryos on the media with 8 mg · L-1 AgNO3，respectively. Low temperature was effective on some accessions to form embryos and calli. 50069 had the embryo ratio of 1% under the treatment of 1 d，and the callus ratio of 11.25% under the treatment of 2 d and 3 d，respectively. 70Q67 had the embryo ratio of 4.00% with 3 d treatment. About 93.39% plants were diploid among 121 plantlets regenerated from embryos with cytogenetic analysis，which was regarded as the result of spontaneous diploidization during anther culture.

Key words: carrot, anther culture, microspore-derived embryo, callus, spontaneous diploidization