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ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (9): 1381-1384.

• 研究报告 • Previous Articles     Next Articles

Polyploid Induction of Nobile-type Dendrobium

ZHENG Bao-qiang1;ZHANG Ying1,2;WANG Yan1*;LI Zhen-jian1;ZHU Xiang-tao1;Lü Chun-yan1   

  1. (1 Research Institute of Forestry, Chinese Academ y of Forestry,Key laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091,China; 2Research Institute of Subtropical Forestry, Chinese Academ y of Forestry, Fuyang, Zhejiang 311400, China)
  • Received:2008-12-11 Revised:2009-06-15 Online:2009-09-25 Published:2009-09-25
  • Contact: WANG Yan

Abstract: A hybrid of nobile-type Dendrobium was treated with colchicines at different concentration and time duration in vitro. The results showed that the best effectwas obtained by the treatment of 0.06% colchicines with 12 hours and its induction rate was 69.1%. Comparing the characteristics of plants and stomas,there were remarkable difference between the treatment and the control. Chromosome observation found that the chromosome number of treated plants was 76 and the chromosome number of the control was 38, indicating the tetraploid (2n = 4x = 76) of nobile-type Dendrobium was induced successfully. The mixer-fold phenomenon was also found among treatment plants.

Key words: Dendrobium, nobile-type Dendrobium, polyploid, colchicine, chromosome

CLC Number: