Abstract: Twenty-three isolates of Botrytis cinerea Pers. were obtained from the diseased tissue collected in Hefei, Bengbu, Changfeng and Hexian, bymeans of tissue isolatingmethod. The pathogenicity of the isolates of B. cinerea from different hosts to the fruits and leaves of strawberrywere investigated by app lyingmycelial block wound inoculation, respectively. The preliminary results showed that all of the tested isolates caused occurrence of greymould on strawberry fruits and leaves, but there be significant difference in the average diameters of the lesions caused by different isolates and there was difference in the different strawberry varieties, suggesting that there was significant differentiation in pathogenicity of B. cinerea to strawberry fruits and leaves among isolates. According to the average diameters of the lesions caused to strawberry fruits and leaves, the pathogenicity of the all isolateswas classified into three categories: strong, intermediate and weak. In general, the isolates from strawberry and tomato were more strongly pathogenic to the fruits and leaves of strawberry, and the isolate from cap sicum was weakly pathogenic to strawberry fruits and leaves. However, there was difference in pathogenicity among the different isolates from the same host, and the pathogenicity difference was not obviously related to the localities of isolates

Key words: Botrytis cinerea, pathogenicity differentiation, strawberry, host