RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (8): 1227-1232.

• 研究简报 • Previous Articles Next Articles

RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight

QIU Yi-peng^1,2;LI Hai-tao^2*;ZHANG Zi-jun²;ZOU Qing-dao^2*

(¹ Horticulture College, Shenyang Agricultural University, Shenyang 110161, China; ² Vegetable Research Institute, LiaoningAcademy of Agricultural Sciences Shenyang 110161, China)

Received:2009-04-15 Revised:2009-06-26 Online:2009-08-25 Published:2009-08-25
Contact: LI Hai-tao;ZOU Qing-dao

Abstract

Abstract: The objective of this studywas to identify RAPD marker for resistance to late blight in tomato.BSA (Bulked SegregantAnalysis) was used to search for RAPD markers linked to late blight resistant gene Ph-3, using F₂ segregating population (147 individuals) derived from a cross of CLN2037 ( resistant) and
T2-03 ( susceptible ). Two hundred and thirty decamer primers with arbitrary sequences are chosen for polymerase chain reaction amplication. One RAPD marker CCPB272-03₇₄₀( the sequence of the primer is GGTCGATCTG) was tightly linked to the resistant gene Ph-3 and it was 518 cM from the resistant gene

Key words: tomato, late blight, RAPD marker, gene Ph-3

CLC Number:

S 641.2

QIU Yi-peng;LI Hai-tao;ZHANG Zi-jun;ZOU Qing-dao. RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight[J]. ACTA HORTICULTURAE SINICA, 2009, 36(8): 1227-1232.

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RAPD Marker of the Resistant Gene Ph-3 for Tomato Late Blight

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