Molecular Cloning and Expression Analysis of Fatty Acid Desaturase Gene in Cucumber

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (9): 1357-1362.

• 研究简报 • Previous Articles Next Articles

Molecular Cloning and Expression Analysis of Fatty Acid Desaturase Gene in Cucumber

LIU Chun-xiang^1*,HE Qi-wei²,ZHAO Guang-qiang¹,KAN Shi-hong¹,and ZHANG Chun-lan¹

(¹Weifang University Weifang Shandong 261061; ² Vegetable Institute of Shandong Academy of Agricultural Science, Jinan 250100, China)

Received:2008-04-22 Revised:2008-05-23 Online:2008-09-25 Published:2008-09-25
Contact: LIU Chun-xiang

Abstract

Abstract: The fatty acid deasturase gene (Cs-FAD), which full-length is 1 807 bp and encode 445 amino acids, was cloned from cucumber leaves by technique of rapid PCR amplification cDNA-ends. High similarity was observed between the sequence of Cs-FAD and FAD of peach ( identity, 77%).The result of RT-PCR simi-quantitative analysis showed that Cs-FAD expresses higher in green tissues, such as leaves, stems, hooks etc. And it could also be found expression in roots, flower, fruits and seeds, in a low level.

Key words: cucumber, gene clone, expression, flavor, resistance

CLC Number:

S 642.2

LIU Chun-xiang;HE Qi-wei;ZHAO Guang-qiang;KAN Shi-hong;and ZHANG Chun-lan. Molecular Cloning and Expression Analysis of Fatty Acid Desaturase Gene in Cucumber [J]. ACTA HORTICULTURAE SINICA, 2008, 35(9): 1357-1362.

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Molecular Cloning and Expression Analysis of Fatty Acid Desaturase Gene in Cucumber

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