Developing of RGA-CAPS markers for resistant gene R10 to potato late blight

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (6): 885-890.

• 研究简报 • Previous Articles Next Articles

Developing of RGA-CAPS markers for resistant gene R10 to potato late blight

WANG Jia-jia^1,2, XU Jian-fei²,LIYing²,WANG Feng-yi¹, HUANG San-wen^2*

（¹College of Agriculture, Northeast Agriculture University, Harbin 150030, China; ²Institute of Vegetable and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China）

Received:2008-02-27 Revised:2008-04-24 Online:2008-06-25 Published:2008-06-25
Contact: HUANG San-wen

Abstract

Abstract: R10 gene conferring resistance to potato (Solanum tuberosum L.) late blight is one of haplotypes in major late blight resistance complex (MLB). Based on sequence analysis for resistant genes known in this complex, it's indicated that these haplotypes have conserved domains and belong to R3a gene family. So twelve primer combinations were designed based on the conserved sequences of R3a gene and its homologs derived from MLB and were used to identify resistance gene analogs (RGA) closely linked to the R10 gene. The conserved primers combinations were screened by a population of the R10 differential (MaR10) and the susceptible potato (Solanum tuberosum) cultivar Katahdin. The PCR products then were digested with twelve restriction enzymes and were assessed for polymorphism by bulk segregant analysis. The RGA-CAPS marker RGA-600 and RGA-1000 linked 0.25 cM to the R10 gene, were developed and made base for the cloning of R10 gene.

Key words: potato, late blight, R3a gene, R10 gene, RGA-CAPS marker

CLC Number:

S 532

WANG Jia-jia;XU Jian-fei;LIYing;WANG Feng-yi;HUANG San-wen. Developing of RGA-CAPS markers for resistant gene R10 to potato late blight[J]. ACTA HORTICULTURAE SINICA, 2008, 35(6): 885-890.

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Developing of RGA-CAPS markers for resistant gene R10 to potato late blight

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