Abstract:

In order to verify the function of StVe gene（3.4 kb）which was isolated from Solanum torvum, we substituted the StVe gene for gus in plasmid p35S-2300 :: gus :: noster, and constructed plant binary expression vector p35S-2300 :: StVe :: noster. Subsequently, the resulting vector was introduced into tomato plants via Agrobacterium tumefaciens-mediated method. In total, we obtained 72 tomato plants resistant to kanamycin and 5 independent transgenic tomato plants were confirmed by PCR and Southern blot analysis. The five transgenic tomato plants were further analyzed the expression of StVe gene at transcription level through semi-quantitative one-step RT-PCR. StVe gene was found to express with variable levels between different transgenic lines. The crude proteins were extracted from the transgenic tomato leaves and proved to have an effect of restraining the mycelia growth of Verticillium dahliae race 1 in vitro.

Key words: Tomato, Resistance to disease, StVe gene, Verticillium dahliae, Transformation