Construction of the Expression Vectors of Tomato LeEIL1 and Their Expression in Engineering Strains

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (5): 687-692.

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Construction of the Expression Vectors of Tomato LeEIL1 and Their Expression in Engineering Strains

LI Ji, LI Zheng-Guo*, LUO An-Cai, YANG Ying-Wu, and DENG Wei

(Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China)

Received:2007-11-05 Revised:2008-02-28 Online:2008-05-25 Published:2008-05-25
Contact: LI Zheng-Guo

Abstract

Abstract:

EIN3 plays an important role in the ethylene signaling pathway of plant. In the study, the tomato LeEIL1 gene was cloned by RT-PCR from tomato fruit, and three expression vectors of tomato LeEIL1 were constructed. After comparision between the two host strains, it was found that the expression of LeEIL1 was more effective when LeEIL1 was cloned in pPIC9k vector and expressed in Pichia pastoris than cloned in pET30a/pET15b vectors while expressed in E.coli BL21(DE3).

Key words: tomato, ethylene-insensitive factor, prokaryotic expression system, eukaryotic expression system

CLC Number:

S 641.2

LI Ji;LI Zheng-Guo;LUO An-Cai;YANG Ying-Wu;and DENG Wei. Construction of the Expression Vectors of Tomato LeEIL1 and Their Expression in Engineering Strains [J]. ACTA HORTICULTURAE SINICA, 2008, 35(5): 687-692.

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Construction of the Expression Vectors of Tomato LeEIL1 and Their Expression in Engineering Strains

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