Abstract:

This paper studied the change of β-EST isoenzymes of small buds (1.5mm or so) in the thermo-sensitive CMS of Chinese Cabbage (Brassica campestris L. ssp. pekinensis) TsCMS7311(A) during the fertility changeover. By one-dimensional GEL electrophoresis analyzing, we found that there were three β-EST isoenzymes related to the sterility changeover closely, their Rf were 0.683, 0.718 and 0.742 respectively. During sterile stamen changed into fertile in male sterile line(AT) after low temperature treatment, the (-EST zymograms changed into maintainer line's(B) too；While fertile stamen in male sterile line (AT) came back into sterile, the (-EST zymograms came back into the natural male sterile line's (A) again. Enriched these β-EST isoenzymes with electroelution, then separated and purified theseβ-EST isoenzymes with two-dimensional GEL electrophoresis, we gained six polypeptides, whose pI values were about 7.7 and molecular weight were between 55 kD and 62 kD.

Key words: Chinese cabbage, sterile changeover, (-EST isoenzymes, two-dimensional GEL electrophoresis