Abstract: The co-dominant AFLP marker of Anthracnose Resistance-related Gene of Cucumber was converted into co-dominant SCAR marker successfully. After sequencing of the AFLP marker fragment, two pairs of primers were designed according to the nucleotide sequence. PCR results from cucumber genomic DNA showed the two primer pairs, (1) and (2), could amplify two band types with the size of 131bp/125 bp and178bp /172 bp respectively. Both 131 bp band and 178 bp band were the character band of anthracnose resistance phenotype. These SCAR markers were designated as SCEM131/125、SCEM178/172。Anthracnose resistance of F2 and some known cucumber lines were tested by SCAR markers, and the results indicated that the resistance tested was consisitent with their field performance by 97.27%, which further confirmed the feasibility of the SCAR markers in MAS for cucumber anthracnose.

Key words: cucumber, anthracnose resistance-related gene, AFLP marker, SCAR marker