AFLP Analysis of New Pear Cultivars and Their Parents

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 847-852.

• 果树 • Previous Articles Next Articles

AFLP Analysis of New Pear Cultivars and Their Parents

WANG Fei;LIN Sheng-hua;FANG Cheng-quan^*;LI Lian-wen

(Research Institute of Pomology, Chinese Academy of Agricultural Sciences, X ingcheng, Liaoning 125100, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: Forty-three pear cultivars including 20 new pear cultivars and their parentswere subjected to
fluorescent AFLP ( amplified fragments length polymorphism) analysis. Seven primer combinations selected
from 64 primer combinations produced a total of 784 fragments, of which 699 were polymorphic with a poly
morphism percentage of 89.2%. The results showed that characteristic bands were amp lified from 29 culti
vars, and any primer combination could identify all the cultivars. Fluorescent AFLP was proved to be a very
reliable method to identify pear cultivars. The genetic relationship between new pear cultivars and their parents
was analyzed and 20 new cultivars were classified on molecular level by clustered analysis, which p rovided
theoretical reference for parent selection in pear hybridization breeding.

Key words: Pyrus, Cultivar, Identification, Fluorescent AFLP, Fingerprinting

WANG Fei;LIN Sheng-hua;FANG Cheng-quan;LI Lian-wen. AFLP Analysis of New Pear Cultivars and Their Parents[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 847-852.

[1]	WANG Rui, HONG Wenjuan, LUO Hua, ZHAO Lina, CHEN Ying, and WANG Jun, . Construction of SSR Fingerprints of Pomegranate Cultivars and Male Parent Identification of Hybrids [J]. Acta Horticulturae Sinica, 2023, 50(2): 265-278.
[2]	REN Hailong, XU Donglin, ZHANG Jing, ZOU Jiwen, LI Guangguang, ZHOU Xianyu, XIAO Wanyu, and SUN Yijia. Establishment of SNP Fingerprinting and Identification of Chinese Flowering Cabbage Varieties Based on KASP Genotyping [J]. Acta Horticulturae Sinica, 2023, 50(2): 307-318.
[3]	LIANG Jiali, WU Qisong, CHEN Guangquan, ZHANG Rong, XU Chunxiang, and FENG Shujie, . Identification of the Neopestalotiopsis musae Pathogen of Banana Leaf Spot Disease [J]. Acta Horticulturae Sinica, 2023, 50(2): 410-420.
[4]	MENG Qiufeng, WANG Bingliang, WANG Yuhong, HUANG Yunping, and WANG Jie. A New Tuber Mustard Cultivar‘Yongzha 805’ [J]. Acta Horticulturae Sinica, 2023, 50(2): 451-452.
[5]	YU Hailong, ZHANG Meiyan, LI Qiaozhen, ZHANG Lujun, SHANG Xiaodong, TAN Qi, ZHOU Feng, and LI Yu. A New Panus giganteus Cultivar‘Shenxun 1’ [J]. Acta Horticulturae Sinica, 2023, 50(2): 453-454.
[6]	WANG Xiqing, JIA Yunhe, YAN Wen, FU Yongkai, YOU Haibo, LI Dongyan, and ZHAO Jingchao. A New Watermelon Cultivar‘Longsheng Jiali’with High Resistance to Fusarium Wilt [J]. Acta Horticulturae Sinica, 2023, 50(2): 455-456.
[7]	ZHU Wei, CAO Jinjin, CHEN Xi, ZHANG Wei, SUN Rongze, ZHU Shaocai, ZHAO Jiageng, CUI Yaqi, WANG Yuxuan, and YU Xiaonan. New Herbaceous Peony Cultivars‘Fluttering Pink’‘Fairy’s Cheek’ ‘Blushing Smile’and‘Tiny Lotus’ [J]. Acta Horticulturae Sinica, 2023, 50(2): 457-458.
[8]	ZHAI Hanhan, ZHAI Yujie, TIAN Yi, ZHANG Ye, YANG Li, WEN Zhiliang, CHEN Haijiang. Genome-wide Identification of Peach SAUR Gene Family and Characterization of PpSAUR5 Gene [J]. Acta Horticulturae Sinica, 2023, 50(1): 1-14.
[9]	JIANG Jingdong, WEI Zhuangmin, WANG Nan, ZHU Chenqiao, YE Junli, XIE Zongzhou, DENG Xiuxin, CHAI Lijun. Exploitation and Identification of Tetraploid Resources of Hongkong Kumquat（Fortunella hindsii） [J]. Acta Horticulturae Sinica, 2023, 50(1): 27-35.
[10]	XING Zhudong, LÜ Futang, GUO Shangjing, ZHANG Yanyi. A New Peach Cultivar‘Liaoda Hongjin’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 225-226.
[11]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, GONG Chao. A New Eggplant Cultivar‘Xinfeng 3’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 227-228.
[12]	CAI Peng, FANG Chao, LI Yuejian, LIU Duchen, LIU Xiaojun, LIANG Genyun. A New Eggplant Cultivar‘Tianjiao’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 229-230.
[13]	ZHANG Hongmei, CUI Jiawei, YU Jizhu, DING Xiaotao, HE Lizhong, JIN Haijun. A New Bitter Gourd Cultivar‘Qingcui 1’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 231-232.
[14]	XU Shenping, YUAN Xiuyun, ZHANG Yan, HE Pei, CUI Bo. A New Dendrobium officinale Cultivar‘Yuhu 4’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 233-234.
[15]	HAN Xiaolei, ZHANG Caixia, LIU Kai, YANG An, YAN Jiadi, LI Wuxing, KANG Liqun, and CONG Peihua. A New Mid-ripening Apple Cultivar‘Zhongping Youlei’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 1-2.

AFLP Analysis of New Pear Cultivars and Their Parents

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments