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ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 1041-1046.

• 研究简报 • Previous Articles     Next Articles

Genetic Diversity of Chrysanthemum Cultivars Revealed by AFLP Analysis

HAN Jie1; HU Nan2; LI Yu-ge1; SHANG Fu-de1*
  

  1. (1 College of Life Sciences and Institute of Agricultural Biotechology, Henan University, Kaifeng, Henan 475001, China;2Nanyang Institute of Technology, Nanyang, Henan 473000, China)
  • Received:2007-05-08 Revised:2007-07-25 Online:2007-08-25 Published:2007-08-25

Abstract: The genetic diversity and relationship of 45 chrysanthemum cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism)-silver staining protocol. AFLP fingerprinting of 45 chrysanthemum cultivars with ten pairs of E+3/M+3 primers revealed a total number of 486 unambiguous bands, of which 451 were polymorphic and 45.1 polymorphic bands per pair of primer were produced on average. The polymorphism frequency was 92.80%. This result showed the abundant diversities of enzyme digestion sites
among used chrysanthemum cultivars. As analyzed by DPSv3.01, the Neips genetic distance of 45 chrysanthe
mum cultivars ranged from 0.36000 to 0.86237, and the average distance was 0.611185. These chrysanthe
mum cultivars were divided into six group s by UPGMA ( unweighted pair group method with arithmetic aver
age) based on Neips genetic distances. The genetic relationship of 45 chrysanthemum cultivars was analyzed
using the different coefficient.

Key words: Chrysanthemum, AFLP, Genetic diversity, Relationship