Abstract:

In this study, the bean iron-binding protein gene (PvFer) was introduced into the genome of tomato Fenhong 908 by Agrobacterium-mediated genetic transformation. The results of PCR，PCR-Southern and Southern blotting analysis confirmed that foreign ferritin gene was integrated into the genome of two tomato plants. RT-PCR revealed that the foreign ferritin gene was expressed in the two tomato plants. Measurement of Fe, Zn, Mn contents in fruit and leaf of the transgenic tomato plants suggested that iron content in leaves of T1 transgenic line was approximately 2.7 times that of the non-transformed leaves, and iron content in fruits was increased by 37%; zinc content in fruits was increased by 119%, but manganese content in fruits of T1 transgenic line was decreased by 56%.

Key words: Tomato, ferritin, Agrobacterium tumefaciens, Transformation